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Pacific Blue™ anti-mouse/human CD45R/B220 Antibody

Pricing & Availability
Clone
RA3-6B2 (See other available formats)
Regulatory Status
RUO
Other Names
B220
Isotype
Rat IgG2a, κ
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Product Citations
publications
RA3-6B2_Blue_091207
C57BL/6 mouse splenocytes stained with Pacific Blue™ RA3-6B2
  • RA3-6B2_Blue_091207
    C57BL/6 mouse splenocytes stained with Pacific Blue™ RA3-6B2
Compare all formats See Pacific Blue™ spectral data
Cat # Size Price Save
103230 25 µg ¥16,740
103227 100 µg ¥38,270
Description

CD45R, also known as B220, is an isoform of CD45. It is a member of the protein tyrosine phosphatase (PTP) family with a molecular weight of approximately 180-240 kD. CD45R is expressed on B cells (at all developmental stages from pro-B cells through mature B cells), activated B cells, and subsets of T and NK cells. CD45R (B220) is also expressed on a subset of abnormal T cells involved in the pathogenesis of systemic autoimmunity in MRL-Faslpr and MRL-Fasgld mice. It plays a critical role in TCR and BCR signaling. The primary ligands for CD45 are galectin-1, CD2, CD3, and CD4. CD45R is commonly used as a pan-B cell marker; however, CD19 may be more appropriate for B cell specificity.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse, Human
Reported Reactivity
Cat
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Abelson murine leukemia virus-induced pre-B tumor cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The suggested use of this reagent is ≤1.0 µg per 106 cells in 100 µl volume. It is highly recommended that the reagent be titrated for optimal performance for each application.

* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Violet Laser (405 nm)
Application Notes

Clone RA3-6B2 has been described to react with an epitope on the extracellular domain of the transmembrane CD45 glycoprotein which is dependent upon the expression of exon A and specific carbohydrate residues. Additional reported applications (for the relevant formats) include: immunoprecipitation1, in vitro and in vivo modulation of B cell responses2-4, immunohistochemistry of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections5,6, and spatial biology (IBEX)14,15.

Application References

(PubMed link indicates BioLegend citation)
  1. Coffman RL. 1982. Immunol. Rev. 69:5. (IP)
  2. George A, et al. 1994. J. Immunol. 152:1014. (Activ)
  3. Asensi V, et al. 1989. Immunology 68:204. (Activ)
  4. Domiati-Saad R, et al. 1993. J. Immunol. 151:5936. (Activ)
  5. Hata H, et al. 2004. J. Clin. Invest. 114:582. (IHC)
  6. Monteith CE, et al. 1996. Can. J. Vet. Res. 60:193. (IHC)
  7. Shih FF, et al. 2006. J. Immunol. 176:3438. (FC)
  8. Chang C L-T, et al. 2007. J. Immunol. 178:6984.
  9. Fazilleau N, et al. 2007. Nature Immunol. 8:753.
  10. Lang GL, et al. 2008. Blood 111:2158. PubMed
  11. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
  12. del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed
  13. Murakami R, et al. 2013. PLoS One. 8:73270. PubMed
  14. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  15. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Wang D, et al. 2022. Cell Rep. 39:110587. PubMed
  2. Grégoire C, et al. 2022. Immunity. 55:1216. PubMed
  3. Tomiaki C, et al. 2022. Front Immunol. 13:1014462. PubMed
  4. Kraemer AN, et al. 2022. Front Immunol. 13:933191. PubMed
  5. Su C, et al. 2022. NPJ Vaccines. 7:168. PubMed
  6. Slusarczyk P, et al. 2023. Elife. 12: . PubMed
  7. Scherer S, et al. 2023. Nat Immunol. 24:501. PubMed
  8. Ferriz M, et al. 2023. STAR Protoc. 4:102079. PubMed
  9. Ke F, et al. 2023. Elife. 12:. PubMed
  10. Chen CC, et al. 2021. J Exp Med. 218:. PubMed
  11. Chappaz S, et al. 2021. Cell Reports. 36(3):109430. PubMed
  12. Schmid C, et al. 2015. J Exp Med. 212:775. PubMed
  13. Yang X, et al. 2020. Blood Sci. 2:89. PubMed
  14. Vijayan K, et al. 2021. Cell Reports. 36(5):109489. PubMed
  15. Makdasi E, et al. 2009. J Immunol. 182:6143. PubMed
  16. Benhamou D, et al. 2018. Front Immunol. 2.260416667. PubMed
  17. Zong L, et al. 2021. NPJ Aging Mech Dis. 7:25. PubMed
  18. Grosjean F, et al. 2015. PLoS One. 10: 0133917. PubMed
  19. Liu X, et al. 2021. eLife. 0.416666666666667. PubMed
  20. Murphy M, et al. 2017. Eur J Immunol. 47:880. PubMed
  21. Hung PJ, et al. 2018. Mol Cell. 71:332. PubMed
  22. Yu AI, et al. 2020. Cell Rep. 107471:31. PubMed
  23. Gajdasik DW, et al. 2020. Nat Commun. 2.834027778. PubMed
  24. Vazquez S, et al. 2015. Exp Hematol. 43: 578-585. PubMed
  25. Ni P, et al. 2014. J Immunol . 193:1778. PubMed
  26. Kat I, et al. 2010. Int Immunol. 0.986805556. PubMed
  27. Chopin M et al. 2018. Immunity. 50(1):77-90 . PubMed
  28. Domeier P, et al. 2016. J Exp Med. 213: 715 - 732. PubMed
  29. Günal-Sadık G, et al. 2014. PLoS One. 9:111748. PubMed
  30. Soni C, et al. 2014. J Immunol. 193:4400. PubMed
  31. Simerska P, et al. 2014. Vaccine. 32:4743. PubMed
  32. Bunn P, et al. 2014. J Immunol. 192:3709. PubMed
  33. Wilder BK, et al. 2022. NPJ Vaccines. 7:58. PubMed
  34. Krishnamurthy B, et al. 2015. Diabetes. 64: 3229-3238. PubMed
  35. Onodera T, et al. 2016. J Immunol. 196: 4172 - 4184. PubMed
  36. Courtney AH et al. 2017. Molecular cell. 67(3):498-511 . PubMed
  37. Jyh Liang Hor, William R. Heath, Scott N. Mueller 2017. Sci Rep. 7:41091. PubMed
  38. Minamitani T, et al. 2015. Proc Natl Acad Sci U S A. 112: 11612-11617. PubMed
  39. Uddback I, et al. 2016. Sci Rep. 6:20137. PubMed
  40. Soni C, et al. 2020. Immunity. 52(6):1022-1038.e7. PubMed
  41. Abbott RK, et al. 2018. Immunity. 48:133. PubMed
  42. Lin J, et al. 2021. Nat Commun. 12:5321. PubMed
  43. Kälin S et al. 2017. Cell metabolism. 26(3):475-492 . PubMed
  44. Chen RJ, et al. 2022. iScience. 25:105595. PubMed
  45. Georgoudaki A, et al. 2016. Cell Rep. 15: 2000-2011. PubMed
  46. Perry JL, et al. 2020. ACS Nano. 5.583333333. PubMed
  47. Alexandre YO, et al. 2020. Cell Reports. 33(13):108567. PubMed
  48. Wang X, et al. 2021. EMBO J. 40:e105926. PubMed
  49. Burgener SS, et al. 2019. Cell Rep. 27:3646. PubMed
  50. Sato K, et al. 2017. J Immunol. 10.4049/jimmunol.1602005. PubMed
  51. Jeljeli M, et al. 2021. Front Immunol. 12:670776. PubMed
  52. Omatsu Y, et al. 2022. Nat Commun. 13:2654. PubMed
  53. Zhang C, et al. 2021. J Immunother Cancer. 9:. PubMed
  54. Miyauchi K, et al. 2016. Nat Immunol. 17:1447-1458. PubMed
  55. Kobia FM, et al. 2020. PLoS Biol. 18:e3000850. PubMed
  56. Bellomo A, et al. 2020. Immunity. 53(1):127-142.e7. PubMed
  57. Teo TH, et al. 2018. Nat Commun. 9:3905. PubMed
  58. Tanaka Y, et al. 2017. J Immunol. 199:4016. PubMed
  59. Raju S, et al. 2020. Cell Reports. 29(9):2556-2564.e3.. PubMed
  60. Miyauchi K, et al. 2021. Nat Commun. 12:3789. PubMed
  61. Lazarian G, et al. 2021. Cancer Cell. 39(3):380-393.e8. PubMed
  62. Sebina I, et al. 2016. PLoS Pathog. 12:e1005999. PubMed
  63. Baglaenko Y, et al. 2016. PLoS One. 11: 0150515. PubMed
  64. Nishimori J, et al. 2012. Infect Immun . 80:4398. PubMed
  65. Cantor DJ et al. 2019. Cell reports. 26(1):108-118 . PubMed
  66. Ilsley M, et al. 2017. Nucleic Acids Research. 10.1093/nar/gkx441. PubMed
  67. Chao JL, et al. 2021. Cell Rep Med. 2:100399. PubMed
  68. McPhee C, et al. 2013. J Immunol. 191:4581. PubMed
  69. Wong E, et al. 2015. J Immunol. 194:4130. PubMed
  70. Kato Y, et al. 2015. J Immunol. 195: 1006-1014. PubMed
  71. Hamilton J,et al. 2017. J Immunol. 10.4049/jimmunol.1700888. PubMed
  72. Gaya M et al. 2018. Cell. 172(3):517-533 . PubMed
  73. Jaitin DA et al. 2016. Cell. 167(7):1883-1896 . PubMed
  74. Smith LK, et al. 2021. Elife. 10:. PubMed
  75. Simoni L, et al. 2020. Cell Rep. 33:108330. PubMed
  76. Baptista M, et al. 2016. Nat Commun. 7:12175. PubMed
  77. Eilat E 2013. J Immunol. 190:1472. PubMed
  78. Schäfer AL, et al. 2021. Front Immunol. 12:696810. PubMed
  79. Fast EM, et al. 2021. Elife. 10:. PubMed
  80. Adachi Y, et al. 2015. J Exp Med. 212: 1709-1723. PubMed
  81. Serr I, et al. 2016. Nat Commun. 7:10991. PubMed
  82. Quijano-Rubio A, et al. 2022. Nat Biotechnol. Online ahead of print. PubMed
  83. Amend A, et al. 2021. Int J Mol Sci. 22:. PubMed
  84. Yoshikawa S, et al. 2016. Sci Rep. 6:18738. PubMed
  85. Carbonetti S et al. 2017. Journal of immunological methods. 448:66-73 . PubMed
RRID
AB_492876 (BioLegend Cat. No. 103230)
AB_492876 (BioLegend Cat. No. 103227)

Antigen Details

Structure
Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution

B cells, T cell subset, NK cell subset

Function
Phosphatase, T and B cell activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4
Cell Type
B cells, NK cells, T cells
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85.
3. Kishihara K, et al. 1993. Cell 74:143.
4. Pulido R, et al. 1988. J. Immunol. 140:3851.

Gene ID
19264 View all products for this Gene ID 5788 View all products for this Gene ID
UniProt
View information about CD45R on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All CD45R/B220 Reagents Request Custom Conjugation
Description Clone Applications
Alexa Fluor® 594 anti-mouse/human CD45R/B220 RA3-6B2 IHC-F,FC,3D IHC
APC anti-mouse/human CD45R/B220 RA3-6B2 FC
Biotin anti-mouse/human CD45R/B220 RA3-6B2 FC,IHC-F
FITC anti-mouse/human CD45R/B220 RA3-6B2 FC
PE anti-mouse/human CD45R/B220 RA3-6B2 FC
PE/Cyanine5 anti-mouse/human CD45R/B220 RA3-6B2 FC
Purified anti-mouse/human CD45R/B220 RA3-6B2 FC,CyTOF®,IHC-F,IHC-P,IP,Activ
PE/Cyanine7 anti-mouse/human CD45R/B220 RA3-6B2 FC
APC/Cyanine7 anti-mouse/human CD45R/B220 RA3-6B2 FC
Alexa Fluor® 488 anti-mouse/human CD45R/B220 RA3-6B2 FC,IHC-F,3D IHC
Alexa Fluor® 647 anti-mouse/human CD45R/B220 RA3-6B2 FC,IHC-F,3D IHC,SB
Pacific Blue™ anti-mouse/human CD45R/B220 RA3-6B2 FC
Alexa Fluor® 700 anti-mouse/human CD45R/B220 RA3-6B2 FC
PerCP anti-mouse/human CD45R/B220 RA3-6B2 FC
PerCP/Cyanine5.5 anti-mouse/human CD45R/B220 RA3-6B2 FC
Brilliant Violet 421™ anti-mouse/human CD45R/B220 RA3-6B2 FC,IHC-F
Brilliant Violet 570™ anti-mouse/human CD45R/B220 RA3-6B2 FC
Brilliant Violet 650™ anti-mouse/human CD45R/B220 RA3-6B2 FC
Brilliant Violet 605™ anti-mouse/human CD45R/B220 RA3-6B2 FC
Brilliant Violet 785™ anti-mouse/human CD45R/B220 RA3-6B2 FC
Brilliant Violet 510™ anti-mouse/human CD45R/B220 RA3-6B2 FC,IHC-F,SB
Purified anti-mouse/human CD45R/B220 (Maxpar® Ready) RA3-6B2 FC,CyTOF®
Brilliant Violet 711™ anti-mouse/human CD45R/B220 RA3-6B2 FC
PE/Dazzle™ 594 anti-mouse/human CD45R/B220 RA3-6B2 FC
APC/Fire™ 750 anti-mouse/human CD45R/B220 RA3-6B2 FC
Brilliant Violet 750™ anti-mouse/human CD45R/B220 RA3-6B2 FC
TotalSeq™-A0103 anti-mouse/human CD45R/B220 RA3-6B2 PG
Spark Blue™ 550 anti-mouse/human CD45R/B220 RA3-6B2 FC
Spark NIR™ 685 anti-mouse/human CD45R/B220 RA3-6B2 FC
TotalSeq™-B0103 anti-mouse/human CD45R/B220 RA3-6B2 PG
Ultra-LEAF™ Purified anti-mouse/human CD45R/B220 RA3-6B2 FC,CyTOF®,IHC-F,IHC-P,IP,Activ
TotalSeq™-C0103 anti-mouse/human CD45R/B220 RA3-6B2 PG
PE/Fire™ 640 anti-mouse/human CD45R/B220 RA3-6B2 FC
APC/Fire™ 810 anti-mouse/human CD45R/B220 RA3-6B2 FC
PE/Fire™ 700 anti-mouse/human CD45R/B220 RA3-6B2 FC
Spark Violet™ 538 anti-mouse/human CD45R/B220 RA3-6B2 FC
Spark YG™ 581 anti-mouse/human CD45R/B220 RA3-6B2 FC
Spark YG™ 570 anti-mouse/human CD45R/B220 RA3-6B2 IHC-F
PE/Fire™ 810 anti-mouse/human CD45R/B220 RA3-6B2 FC
Spark Blue™ 574 anti-mouse/human CD45R/B220 Antibody RA3-6B2 FC
Spark Violet™ 423 anti-mouse/human CD45R/B220 Antibody RA3-6B2 FC
Spark Red™ 718 anti-mouse/human CD45R/B220 RA3-6B2 FC
Spark UV™ 387 anti-mouse/human CD45R/B220 RA3-6B2 FC
Spark PLUS UV395™ anti-mouse/human CD45R/B220 RA3-6B2 FC
Go To Top Version: 2    Revision Date: 04/25/2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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