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PE anti-ASC (TMS-1) Antibody

Pricing & Availability
Clone
HASC-71 (See other available formats)
Regulatory Status
RUO
Other Names
PYD and CARD domain-containing protein, apoptosis-associated speck-like protein containing a CARD, caspase recruitment domain protein 5, target of methylation-induced silencing (TMS1), CARD5
Isotype
Mouse IgG1, κ
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Product Citations
publications
HASC-71_PE_ASC_Antibody_ICFC_120414
Human acute promyelocytic leukemia cell line, HL-60, was treated with Fixation Buffer and Permeabilization Wash Buffer, and then was stained with anti-human ASC (clone HASC-71) PE (filled histogram) or mouse IgG1, κ PE isotype control (open histogram).
  • HASC-71_PE_ASC_Antibody_ICFC_120414
    Human acute promyelocytic leukemia cell line, HL-60, was treated with Fixation Buffer and Permeabilization Wash Buffer, and then was stained with anti-human ASC (clone HASC-71) PE (filled histogram) or mouse IgG1, κ PE isotype control (open histogram).
See PE spectral data
Cat # Size Price Save
653903 25 tests ¥36,300
653904 100 tests ¥84,480
Description

Apoptosis-associated speck-like protein containing a CARD (ASC), also known as target of methylation-mediated silencing (TMS1) and PYD and CARD domain containing protein (PYCARD), is a 22 kD pro-apoptotic protein containing an N-terminal pyrin domain (PYD) and a C-terminal caspase recruitment domain (CARD). The TMS1 gene was originally found to be aberrantly methylated and silenced in various cancer cells. Expression of ASC can be induced by pro-apoptotic/inflammatory stimuli. In normal cells, this protein is localized to the cytoplasm; however, in cells undergoing apoptosis, it forms ball-like aggregates near the nuclear periphery. During apoptosis, ASC translocated from the cytosol to the mitochondria and associates with mitochondrial Bax to trigger cytochrome c release and subsequent apoptosis. The adaptor molecule ASC mediates AIM2-dependent caspase-1 activation. Recent studies also show that AIM2/ASC complex acts as a novel caspase-8 activation platform. Moreover, emerging reports show novel roles for ASC in disease conditions such as arthritis.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human ASC (aa 113-195).
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Imamura R, et al. 2010. J. Immunol. 184:5874. (WB, IP)
  2. Pierini R, et al. 2012. Cell Death Differ. 19:1709.
Product Citations
  1. Hurtado-Navarro L, et al. 2022. Methods Mol Biol. 2459:1. PubMed
  2. Ruder AV, et al. 2023. Front Immunol. 14:1078591. PubMed
  3. Malhotra S, et al. 2023. Neurol Neuroimmunol Neuroinflamm. 10:. PubMed
  4. Piancone F, et al. 2021. Front Neurosci. 15:639646. PubMed
  5. Bambouskova M, et al. 2021. Cell Reports. 34(10):108756. PubMed
  6. Heiser D, et al. 2021. PLoS One. 16:e0248668. PubMed
  7. Lawless D, et al. 2018. Front Immunol. 9:1527. PubMed
  8. Scambler T et al. 2019. Elife. 8 pii: e49248. PubMed
RRID
AB_2564507 (BioLegend Cat. No. 653903)
AB_2564507 (BioLegend Cat. No. 653904)

Antigen Details

Structure
195 amino acids with a predicted molecular weight of 21 kD.
Distribution

Cytoplasm

Function
ASC promotes caspase-mediated apoptosis. This pro-apoptotic activity is mediated predominantly through the activation of caspase-9. ASC is an integral component of the inflammasome. Within the inflammasome ASC links caspase-1 and NOD-like receptors (NLR) leading to the activation of caspase-1.
Interaction
Interacts with CIAS1/PYPAF1 and PYDC1.
Cell Type
B cells
Biology Area
Cell Biology, Immunology, Neuroscience
Antigen References

1. Masumoto J, et al. 1999. J. Biol. Chem. 274:33835.
2. Conway KE, et al. 2000. Cancer Res. 60:6236.
3. Strong R, et al. 1991. Brain Res. 542:23.
4. Ohtsuka T, et al. 2004. Nat. Cell Biol. 6:121.
5. Srinivasula SM, et al. 2002. J. Biol. Chem. 277:21119.
6. Ippagunta SK, et al. 2010. J. Biol. Chem. 285:12454.

Gene ID
29108 View all products for this Gene ID
UniProt
View information about ASC on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 12/08/2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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