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- Clone
- 9B5A13 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- B-cell-activating transcription factor, Basic leucine zipper transcriptional factor ATF-like, SFA-2, BATF1
- Isotype
- Mouse IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
CD3/CD28-stimulated (3 days) human peripheral blood lymphocytes were treated with FOXP3 Fix/Perm Buffer Set and then intracellularly stained with BATF (clone 9B5A13) PE (filled histogram). Open histogram represents staining with non-stimulated cells.
| Cat # | Size | Price | Save |
|---|---|---|---|
| 654803 | 25 tests | ¥33,660 | |
| 654804 | 100 tests | ¥84,480 |
BATF is a basic leucine zipper transcription factor belonging to the ATF superfamily. BATF forms heterodimers with Jun proteins, which in turn binds to AP-1 consensus sites. The BATF/Jun heterodimers have significantly reduced transcriptional activity compared to Fos/Jun heterodimers. Therefore, induction of BATF expression suppresses AP-1 target genes by competing for Fos/Jun binding sequences. BATF is expressed at low levels in hematopoietic cells and can be induced in response to a variety of stimuli. BATF knockout mice show defects in cytokine expression and in the differentiation of T helper cell subsets and B cells, but not in the proliferation of lymphocytes. These findings suggest that BATF is a tissue-specific regulator for AP-1 activity and plays an essential role in the regulation of immune responses. During early EBV infection, BATF expression is induced by EBNA2 proteins, subsequently preventing infected B cells from entering apoptotic and lytic pathways. This indicates that BATF functions as a modulator to maintain latency status of virus-infected cells.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Full length human BATF recombinant protein.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
ICFC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
- Excitation Laser
-
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Product Citations
-
- RRID
-
AB_2563517 (BioLegend Cat. No. 654803)
AB_2563517 (BioLegend Cat. No. 654804)
Antigen Details
- Structure
- 14 kD protein containing a bZIP (basic-leucine zipper) domain, which mediates dimerization with Jun family members.
- Distribution
-
Nucleus. Expressed most abundantly in hematopoietic tissues.
- Function
- AP-1 transcription complexes regulate gene expression in response to various stimuli, such as cytokines, growth factors, stress, and infection. BATF belongs to the AP-1/ATF superfamily and negatively regulates AP-1 mediated transcription when it forms heterodimers with Jun proteins.
- Interaction
- Forms heterodimers with c-Jun, JunB, and JunD. Interacts with IFI35, IRF4, and IRF8 through bZIP domain.
- Cell Type
- B cells, Dendritic cells
- Biology Area
- Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Transcription Factors
- Molecular Family
- Nuclear Markers
- Antigen References
-
1. Betz BC, et al. 2010. J. Exp. Med. 207:933.
2. Dorsey MJ, et al. 1995. Oncogene 11:2255.
3. Echlin DR, et al. 2000. Oncogene 19:1752.
4. Johansen LM, et al. 2003. J. Virol. 77:6029.
5. Schraml BU, et al. 2009. Nature 460:405.
6. Tussiwand R, et al. 2012. Nature 490:502. - Gene ID
- 10538 View all products for this Gene ID
- UniProt
- View information about BATF on UniProt.org
Related Pages & Pathways
Pathways
Related FAQs
- What type of PE do you use in your conjugates?
- We use R-PE in our conjugates.
Other Formats
View All BATF Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| PE anti-BATF | 9B5A13 | ICFC |
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Compare Data Across All Formats
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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