- Clone
- 1D4-C5 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Vitamin B7, Vitamin H
- Isotype
- Mouse IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Save |
---|---|---|---|
409003 | 25 µg | ¥17,600 | |
409004 | 100 µg | ¥47,080 |
Biotin (vitamin B7 or vitamin H) is essential for cell growth and the metabolization of fat and protein. It is also often used to tag a molecule of interest. The anti-biotin antibody can be used as a second-step reagent for detecting biotinylated targets.
Product DetailsProduct Details
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Biotinylated limulus hemocyanin
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.06 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
-
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
-
Additional reported applications (for relevant formats) include: immunoprecipitation and ELISA.
-
Application References
(PubMed link indicates BioLegend citation) -
- Uccellini MB, et al. 2008. J. Immunol. 181:5875.
- Green NM, et al. 2012. J Biol Chem. 287:39789. PubMed.
- Product Citations
-
- RRID
-
AB_10641847 (BioLegend Cat. No. 409003)
AB_10641847 (BioLegend Cat. No. 409004)
Antigen Details
- Ligand/Receptor
- Avidin, Streptavidin
- Gene ID
- NA
- UniProt
- View information about Biotin on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
- What type of PE do you use in your conjugates?
- We use R-PE in our conjugates.
Other Formats
View All Biotin Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-Biotin | 1D4-C5 | FC,IP,ELISA |
PE anti-Biotin | 1D4-C5 | FC |
Purified anti-Biotin (Maxpar® Ready) | 1D4-C5 | FC,CyTOF® |
TotalSeq™-A0436 anti-Biotin | 1D4-C5 | PG |
Ultra-LEAF™ Purified anti-Biotin | 1D4-C5 | FC,IP,ELISA |
TotalSeq™-C0436 anti-Biotin | 1D4-C5 | PG |
Customers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
-
Purified anti-Biotin
C57BL/6 thymocytes stained with biotinylated CD4 (GK1.5), fo... -
PE anti-Biotin
C57BL/6 thymocytes stained with biotinylated CD4 (GK1.5), fo... -
Purified anti-Biotin (Maxpar® Ready)
Human PBMCs stained with Biotin-anti Human CD3 (UCHT1) then ... -
TotalSeq™-A0436 anti-Biotin
-
Ultra-LEAF™ Purified anti-Biotin
C57BL/6 thymocytes stained with biotinylated CD4 (GK1.5), fo... -
TotalSeq™-C0436 anti-Biotin
Follow Us