PE anti-GFP Antibody anti-GFP

Clone: FM264G (See other available formats)
Regulatory Status: RUO
Other Names: Green Fluorescent Protein
Isotype: Rat IgG2a, κ
Ave. Rating: Submit a Review
Product Citations: publications

FM264G_PE_GFP_Antibody_FC_011017 — GFP-transfected CHO cells were fixed and permeabilized, and then intracellularly stained with anti-GFP (clone FM264G) PE (filled histogram) or rat IgG2a, κ PE isotype control (open histogram).

Compare all formats See PE spectral data

Cat #	Size	Price	Save
338003	25 tests	¥32,120
338004	100 tests	¥69,740

Description

Green fluorescent protein (GFP) was originally identified as a protein involved in bioluminescence, which is from the jellyfish Aequorea Victoria. It is widely used as a fluorescent indicator for monitoring gene expression in a variety of cellular systems, including living organisms and fixed tissues. Unlike other bioluminescent reporters, GFP fluoresces without the need for exogenous substrates or cofactors, or other intrinsic or extrinsic proteins, making GFP a useful tool for monitoring gene expression and protein localization in vivo. Purified GFP is a 27 kD monomer consisting of 238 amino acids and emits green light (emission maximum at 509 nm) when excited with blue or UV light.

Product Details

Technical data sheet

Product Details

Antibody Type: Monoclonal
Host Species: Rat
Immunogen: TLR9-GFP transfected cell line
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunocytochemistry¹.
RRID: AB_2650614 (BioLegend Cat. No. 338003)
AB_2650614 (BioLegend Cat. No. 338004)

Antigen Details

Biology Area: Cell Biology, Immunology
Antigen References: 1. Ishikura H, et al. 2004. Anticancer Res. 24:719.
2. Rizzuto R, et al. 1996. Curr. Biol. 6:183.
3. Chalfie M, et al. 1994. Science 263:802.
Gene ID: NA
UniProt: View information about GFP on UniProt.org

Documentation

Reviews

Review This Product

Related Protocols

Related Products

Description	Clone	Applications

Related Pages & Pathways

Related FAQs

What type of PE do you use in your conjugates?: We use R-PE in our conjugates.
Can I use common compensation control for GFP, CFSE and FITC because they emit in the same channel?: It is not recommended even if they emit in the same channel because these are still different fluors with different brightness intensities. Individual compensation controls should be employed.

Other Formats

View All GFP Reagents Request Custom Conjugation

Description	Clone	Applications
Alexa Fluor® 488 anti-GFP	FM264G	ICFC,ICC
Purified anti-GFP	FM264G	ICFC,ICC
PE anti-GFP	FM264G	ICFC
Alexa Fluor® 647 anti-GFP	FM264G	ICFC,ICC
APC anti-GFP	FM264G	ICFC
PerCP/Cyanine5.5 anti-GFP	FM264G	ICFC
PE/Cyanine7 anti-GFP	FM264G	ICFC

Certificate of Analysis

Customers Also Purchased

Alexa Fluor® 488 anti-GFP

Clone: FM264G

Alexa Fluor® 647 anti-GFP

Clone: FM264G

APC anti-GFP

Clone: FM264G

Ultra-LEAF™ Purified anti-human CD3

Clone: OKT3

Go To Top Version: 2 Revision Date: 12/16/2021

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.