PE anti-human CD137L 4-1BB Ligand Antibody anti-4-1BB Ligand

Clone: 5F4 (See other available formats)
Regulatory Status: RUO
Other Names: 4-1BBL, CD137L, TNFSF9, 41BBL
Isotype: Mouse IgG1, κ
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Product Citations: publications

5F4_PE_071007 — Human T lymphoma cell line HUT-78 stained with 5F4 PE

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Cat #	Size	Price	Save
311503	25 tests	¥31,020
311504	100 tests	¥61,600

Description

4-1BB ligand, also known as CD137L, is a 97 kD member of the TNF superfamily mainly expressed on APCs, activated B and T cells. It has been reported to be important in T cell proliferation and cytokine production through interaction with 4-1BB receptor. 4-1BB ligand appears to be able to act as a costimulatory molecule without the engagement of other costimulatory molecules such as CD28.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Human
Reported Reactivity: African Green, Baboon, Cynomolgus, Rhesus
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 311504) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated anti-mouse IgG second step (Cat. No. 405303), followed by SAv-PE (Cat. No. 405204)).
RRID: AB_314882 (BioLegend Cat. No. 311503)
AB_314882 (BioLegend Cat. No. 311504)

Antigen Details

Structure: TNF superfamily, 97 kD
Distribution: Antigen presenting cells, activated B cells and T cells
Function: T cell proliferation, cytokine production
Ligand/Receptor: 4-1BB (CDw137)
Cell Type: Antigen-presenting cells, B cells, T cells
Biology Area: Costimulatory Molecules, Immunology
Molecular Family: CD Molecules
Antigen References: 1. Akiba H, et al. 2000. J. Exp. Med. 191:375.
2. Pollak KE, et al. 1995. Eur. J. Immunol. 25:488.
3. DeBenedette MA, et al. 1997. J. Immunol. 158:551.
4. Goodwin RG, et al. 1993. Eur. J. Immunol. 23:2631.
Gene ID: 8744 View all products for this Gene ID
UniProt: View information about CD137L on UniProt.org

Documentation

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Description	Clone	Applications

Related Pages & Pathways

Pathways

TNF Superfamily Pathway

Related FAQs

What type of PE do you use in your conjugates?: We use R-PE in our conjugates.

Other Formats

View All CD137L Reagents Request Custom Conjugation

Description	Clone	Applications
PE anti-human CD137L (4-1BB Ligand)	5F4	FC
Purified anti-human CD137L (4-1BB Ligand)	5F4	FC
APC anti-human CD137L (4-1BB Ligand)	5F4	FC
Brilliant Violet 421™ anti-human CD137L (4-1BB Ligand)	5F4	FC
PE/Dazzle™ 594 anti-human CD137L (4-1BB Ligand)	5F4	FC
PerCP/Cyanine5.5 anti-human CD137L (4-1BB Ligand)	5F4	FC
APC/Fire™ 750 anti-human CD137L (4-1BB Ligand)	5F4	FC
PE/Cyanine7 anti-human CD137L (4-1BB Ligand)	5F4	FC
TotalSeq™-C0022 anti-human CD137L (4-1BB Ligand)	5F4	PG
TotalSeq™-B0022 anti-human CD137L (4-1BB Ligand)	5F4	PG

Certificate of Analysis

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Go To Top Version: 2 Revision Date: 01/29/2018

For Research Use Only. Not for diagnostic or therapeutic use.

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