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PE anti-human CD16 Recombinant Antibody

Pricing & Availability
Clone
B73.1.Rec (See other available formats)
Regulatory Status
RUO
Other Names
FcγRIII, IGFR3, FCG3, FCGR3, FCGRIII, Fc gamma receptor, Fc gamma receptor 3
Isotype
Mouse IgG1, κ
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Product Citations
publications
B731-Rec_PE_CD16_Antibody_1_020625-3
Human peripheral blood lymphocytes were stained with anti-human CD56 (clone HCD56) Brilliant Violet 421™ and anti-human CD16 recombinant (clone B73.1. Rec) PE antibody (left), or mouse IgG1, κ PE isotype control (right).
  • B731-Rec_PE_CD16_Antibody_1_020625-3
    Human peripheral blood lymphocytes were stained with anti-human CD56 (clone HCD56) Brilliant Violet 421™ and anti-human CD16 recombinant (clone B73.1. Rec) PE antibody (left), or mouse IgG1, κ PE isotype control (right).
See PE spectral data
Cat # Size Price Save
360739 25 tests ¥25,740
360740 100 tests ¥52,360
Description

CD16 is known as low affinity IgG receptor III (FcγRIII). It is expressed as two distinct forms (CD16a and CD16b). CD16a (FcγRIIIA) is a 50-65 kD polypeptide-anchored transmembrane protein. It is expressed on the surface of NK cells, activated monocytes, macrophages, and placental trophoblasts in humans. CD16b (FcγRIIIB) is a 48 kD glycosylphosphatidylinositol (GPI)-anchored protein. Its extracellular domain is over 95% homologous to that of CD16a, and it is expressed specifically on neutrophils. CD16 binds aggregated IgG or IgG-antigen complex which functions in NK cell activation, phagocytosis, and antibody-dependent cell-mediated cytotoxicity (ADCC).

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Recombinant
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)

Antigen Details

Structure
Ig superfamily, transmembrane form (50-65 kD) or GPI-linked form (48 kD)
Distribution

NK cells, activated monocytes, macrophages, neutrophils and a subset of T cells

Function
Low affinity IgG Fc receptor, phagocytosis, ADCC
Interaction
CD16 binds aggregated IgG or IgG-antigen complex which functions in NK cell activation, phagocytosis, and antibody-dependent cell-mediated cytotoxicity (ADCC)
Ligand/Receptor
IgG Fc receptor III (FcγRIII)
Cell Type
Macrophages, Monocytes, Neutrophils, NK cells, T cells
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules, Fc Receptors
Antigen References
  1. Rataj F, et al. 2019. Br J Cancer. 120:79-87.
  2. Kaufmann M, et al. 2021. Med. 2:296-312.
  3. Sigal N, et al. 2022. Methods Mol Biol. 2543:113-128. 
  4. Cheung P, et al. 2018. Cell. 173:1385. 
  5. Prodjinotho UF, et al. 2017. PLoS Negl Trop Dis. 11:e0005777. 
  6. Roussel M, et al. 2021. Cell Reports Medicine. 2:100291. 
  7. Waugh KA, et al. 2020. Cell Reports. 29:1893-1908.e4.
Gene ID
2214 View all products for this Gene ID 2215 View all products for this Gene ID
UniProt
View information about CD16 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 02/06/2025

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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