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- Clone
- UP-D2 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- CD300LF, IREM-1, IgSF13,
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
Human peripheral blood monocytes stained with UP-D2 PE
IREM-1 (immune receptor expressed by myeloid cell 1) is a type I single transmembrane protein encoded by human chromosome 17q24.25. A member of the CD300 (CMRF-35) family and the Ig superfamily, IREM-1 is also known as CD300F, or CD300LF (CD300-like member F). CD300F is expressed on monocytes, granulocytes, and myeloid leukemia cell lines. The cytoplasmic domain of IREM-1 contains an ITIM and associates with the P85α subunit of PI3K (phosphoinositide 3-kinase). IREM-1 also functions as an inhibitory receptor.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
- Excitation Laser
-
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
-
Additional reported applications include: Coengagement of IREM-1 with UP-D2 inhibits FcReI-mediated degranulation, immunoprecipitation
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Application References
(PubMed link indicates BioLegend citation) -
- Alvarez-Errico D, et al. 2004. Eur. J. Immunol. 34:3690
- Lee SM, et al. 2011. J. Immunol. 186:6296. PubMed.
- RRID
-
AB_2074121 (BioLegend Cat. No. 340604)
Antigen Details
- Structure
- Type I single transmembrane protein with cytoplamic ITIM, associates with p85α of PI3K, Ig superfamily
- Distribution
-
Monocytes, granulocytes, and myeloid leukemia cell lines
- Function
- Inhibition of myeloid function
- Cell Type
- Monocytes, Granulocytes, Leukemia
- Biology Area
- Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
1. Marquez JA, et al. 2007. J. Mol. Bio. 367:310
2. Alvarez-Errico D, et al. 2004. Eur. J. Immunol. 34:3690
3. Alvarez-Errico D, et al. 2007. J. Immunol. 178:808 - Gene ID
- 146722 View all products for this Gene ID
- UniProt
- View information about CD300F on UniProt.org
Related FAQs
- What type of PE do you use in your conjugates?
- We use R-PE in our conjugates.
Other Formats
View All CD300F Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| PE anti-human CD300F (IREM-1) | UP-D2 | FC |
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Compare Data Across All Formats
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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PE anti-human CD300F (IREM-1)
Human peripheral blood monocytes stained with UP-D2 PE
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