- Clone
- TREM-26 (See other available formats)
- Regulatory Status
- RUO
- Workshop
- HCDM listed
- Other Names
- Triggering receptor expressed on myeloid cells 1, triggering receptor expressed on monocytes 1
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![TREM-26_PE_020708 TREM-26_PE_020708](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/TREM-26_PE_020708.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Human peripheral blood granulocytes stained with TREM-26 PE
TREM-1 is a 30 kD glycoprotein also known as triggering receptor expressed on myeloid cells 1, and triggering receptor expressed on monocytes 1. It is a Type I membrane protein that contains an immunoglobulin-like V-type domain. Alternatively spliced protein variant may be secreted. TREM-1 is highly expressed on peripheral blood myeloid cells (particularly mature monocytes and granulocytes); TREM-1 expression can be further upregulated by bacteria, fungi and lipopolysaccharide. TREM-1 has been shown to interact with the adaptor protein DAP12 to stimulate neutrophil and monocyte-mediated inflammatory responses through the triggering and release of pro-inflammatory cytokines and chemokines. TREM-1 is thought to amplify inflammatory responses to fungal and bacterial infections and potentiate septic shock. This antibody has been shown to be useful for flow cytometry and activation of monocytes and granulocytes.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Reported Reactivity
- African Green, Baboon, Cynomolgus, Rhesus
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Recombinant human TREM-1
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
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FC - Quality tested
- Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
- Excitation Laser
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Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
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ELISA Detection: The biotinylated TREM-26 antibody is useful as a detection antibody for a TREM-1 specific sandwich ELISA, when used in conjunction with the TREM-37 antibody (Cat. No. 316102) as the capture antibody. The TREM-26 antibody is useful for flow cytometry and activation of monocytes and granulocytes. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 314913 & 314914).
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Application References
(PubMed link indicates BioLegend citation) -
- Li J, et al. 2011. Dev. Comp. Immunol. epub.
- Product Citations
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- RRID
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AB_389355 (BioLegend Cat. No. 314906)
Antigen Details
- Structure
- Type I membrane protein, 30 kD, contains immunoglobulin-like V-type domain. Alternatively spliced variant may be secreted.
- Distribution
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Highly expressed in adult liver, lung, and spleen, lower expression in lymph node, placenta, spinal cord, and heart. High expression in peripheral blood myeloid cells.
- Function
- Stimulates neutrophil and monocyte-mediated inflammatory responses through the triggering and release of pro-inflammatory cytokines and chemokines and the upregulation of surface activation markers. Amplifies inflammatory responses to fungal and bacterial
- Interaction
- DAP12
- Modification
- Glycosylated
- Cell Type
- Neutrophils
- Biology Area
- Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
- Molecular Family
- CD Molecules, Cytokines/Chemokines
- Antigen References
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1. Bouchon A, et al. 2001. Nature 410:1103.
2. Bouchon A, et al. 2000. J. Immunol. 164:4991.
3. Gingras MC, et al. 2002. Mol. Immunol. 38:817. - Regulation
- Upregulated by bacteria, fungi, and LPS
- Gene ID
- 54210 View all products for this Gene ID
- UniProt
- View information about CD354 on UniProt.org
Related FAQs
- What type of PE do you use in your conjugates?
- We use R-PE in our conjugates.
Other Formats
View All CD354 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-human CD354 (TREM-1) | TREM-26 | FC,WB |
Biotin anti-human CD354 (TREM-1) | TREM-26 | FC,ELISA Detection |
PE anti-human CD354 (TREM-1) | TREM-26 | FC |
APC anti-human CD354 (TREM-1) | TREM-26 | FC |
Ultra-LEAF™ Purified anti-human CD354 (TREM-1) | TREM-26 | FC,Activ,WB |
TotalSeq™-A0586 anti-human CD354 (TREM-1) | TREM-26 | PG |
TotalSeq™-C0586 anti-human CD354 (TREM-1) Antibody | TREM-26 | PG |
TotalSeq™-B0586 anti-human CD354 (TREM-1) | TREM-26 | PG |
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human CD354 (TREM-1)
Recombinant human TREM-1 Fc protein (20 ng per lane) was res... Recombinant human TREM-1 Fc protein (20 ng per lane) was res... -
Biotin anti-human CD354 (TREM-1)
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PE anti-human CD354 (TREM-1)
Human peripheral blood granulocytes stained with TREM-26 PE -
APC anti-human CD354 (TREM-1)
Human peripheral blood granulocytes stained with TREM-26 APC -
Ultra-LEAF™ Purified anti-human CD354 (TREM-1)
Human peripheral blood granulocytes stained with purified TR... -
TotalSeq™-A0586 anti-human CD354 (TREM-1)
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TotalSeq™-C0586 anti-human CD354 (TREM-1) Antibody
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TotalSeq™-B0586 anti-human CD354 (TREM-1)
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