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PE anti-human EGFRvIII Recombinant Antibody

Pricing & Availability
Clone
DH8.3.Rec (See other available formats)
Regulatory Status
RUO
Other Names
EGFRv3
Isotype
Mouse IgG1, κ
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Product Citations
publications
DH83Rec_PE_EGFRvIII_FC_100924
Human glioblastoma cell line, DKMG/EGFRvIII, was stained with anti-human EGFRvIII recombinant (clone DH8.3.Rec) PE (filled histogram) or mouse IgG1, κ PE isotype control (open histogram).
  • DH83Rec_PE_EGFRvIII_FC_100924
    Human glioblastoma cell line, DKMG/EGFRvIII, was stained with anti-human EGFRvIII recombinant (clone DH8.3.Rec) PE (filled histogram) or mouse IgG1, κ PE isotype control (open histogram).
See PE spectral data
Cat # Size Price Save
335613 25 tests ¥33,660
335614 100 tests ¥75,020
Description

Epidermal growth factor receptor variant III (EGFRvIII) arises from an in-frame deletion of exons 2 to 7 in the extracellular region of EGFR, resulting in a truncated receptor that is constitutively active. This mutation is cancer-specific and is present in approximately 50% of glioblastoma tumors. 

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Recombinant
Host Species
Mouse
Immunogen
Peptide LEEKKGNYVVTDHC conjugated to KLH
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)

Antigen Details

Structure
Deletion of exons 2-7 of the EGFR gene. Molecular mass of approximately 145 kD.
Distribution

Present in approximately 50% of glioblastoma tumors

Function
Pro-tumorigenic (promotes tumor growth, survival, invasion, stemness, metabolism, and angiogenesis).
Biology Area
Cancer Biomarkers
Antigen References
  1. Hui G, et al. 2013. FEBS Journal. 280:21.
Gene ID
1956 View all products for this Gene ID
UniProt
View information about EGFRvIII on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 10/09/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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