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PE anti-human EOS Antibody

Pricing & Availability
Clone
W16032B (See other available formats)
Regulatory Status
RUO
Other Names
Ikaros family zinc finger protein 4 (IKZF4), ZMFN1A4, KIAA1782
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W16032B_PE_EOS_Antibody_010920.png
Human peripheral blood lymphocytes were surface stained with CD3 FITC and CD4 APC/Fire™ 750 and then treated with the True-Nuclear™ Transcription Factor Buffer Set. Cells were then stained with PE EOS (clone W16032B) (left) or rat IgG2a, κ isotype PE (right), and FOXP3 (clone 206D) Alexa Fluor® 647. Dot plots are gated on CD3+CD4+ cells.
  • W16032B_PE_EOS_Antibody_010920.png
    Human peripheral blood lymphocytes were surface stained with CD3 FITC and CD4 APC/Fire™ 750 and then treated with the True-Nuclear™ Transcription Factor Buffer Set. Cells were then stained with PE EOS (clone W16032B) (left) or rat IgG2a, κ isotype PE (right), and FOXP3 (clone 206D) Alexa Fluor® 647. Dot plots are gated on CD3+CD4+ cells.
See PE spectral data
Cat # Size Price Save
397403 25 tests ¥33,660
397404 100 tests ¥87,120
Description

EOS also known as IKZF4 is an IKAROS Family Member expressed in lymphocytes, specifically in Tregs. It plays an important role in the inhibitory function of Tregs. It may be involved in the development of the central and peripheral nervous systems as well as lymphoid development.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Human EOS recombinant protein (271-520 a.a.)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
RRID
AB_2832773 (BioLegend Cat. No. 397403)
AB_2832773 (BioLegend Cat. No. 397404)

Antigen Details

Structure
IKAROS family member
Distribution

Lymphocytes, specifically in Tregs

Function
Essential for inhibitory function of Tregs
Interaction
Binds to 5'GGGAATRCC-3' Ikaros-binding sequence
Antigen References
  1. Honma Y, et al. 1999. FEBS Lett. 447:76-80. 
  2. Perdomo J, et al. 2000. J. Biol. Chem. 275:38347-54. 
  3. Westman BJ, et al. 2004. Biochemistry. 43:13318-27. 
  4. Pan F, et al. 2009. Science. 10.1126/science.1176077. 
Gene ID
64375 View all products for this Gene ID
UniProt
View information about EOS on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 01/09/2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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