PE anti-human Folate Receptors alpha and beta FR-alphabeta Antibody anti-FRaB

Clone: No.5/FOLR (See other available formats)
Regulatory Status: RUO
Other Names: FR-αβ
Isotype: Rat IgG2a, κ
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No5slashFOLR_PE_Folate_Antibody_1_011218 — Human epidermal carcinoma cells (KB cell line) were stained with PE anti-human FR-αβ (clone No.5/FOLR) (filled histogram) or PE Rat IgG2a, κ isotype control (open histogram).

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Cat #	Size	Price	Save
391805	25 tests	¥31,020
391806	100 tests	¥79,200

Description

Human folate receptors alpha and beta (FR-α and FR-β) are members of a family of folate binding receptors that have diverse structural identities but mediate transport of folates into cells. The FR-α and FR-β isoforms are both GPI-anchored proteins with high affinity for folic acid. It is postulated that these receptors function as folate scavengers when folate supply is low or rapid cell growth requires elevated uptake of folate for methylation reactions including DNA biosynthesis. The expression of FR-α and FR-β are distinct in normal and malignant tissues. In normal tissue, FR-α is mainly expressed on the surface of a subset of polarized epithelial cells whereas its aberrant expression has been prominently correlated with malignancies of epithelial origin. Although the FR-β protein was originally thought to be specific to placenta, it can also exist in other tissues, and it may play a role in the transport of methotrexate in synovial macrophages in rheumatoid arthritis patients.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Human
Reported Reactivity: Monkey
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Human Folate beta transfected cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: This clone reacts with both Human FOLR1 and FOLR2
RRID: AB_2721337 (BioLegend Cat. No. 391805)
AB_2721337 (BioLegend Cat. No. 391806)

Antigen Details

Structure: 30 kD, seven disulfide bonds
Distribution: Epithelial cells and same type of tumor cells
Function: Transport of folate into cells
Ligand/Receptor: Folic Acid
Cell Type: Epithelial cells
Biology Area: Cancer Biomarkers, Cell Biology, Immunology
Antigen References: 1. Elwood PC, 1989. J Biol Chem. 264:14893
2. Shen F, et al. 1994. Biochemistry. 33:1209-15
3. Yi YS, 2016. Immune Netw. 16:337
Gene ID: 2348 View all products for this Gene ID 2350 View all products for this Gene ID
UniProt: View information about FR-alphabeta on UniProt.org

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Description	Clone	Applications

Related Pages & Pathways

Related FAQs

What type of PE do you use in your conjugates?: We use R-PE in our conjugates.

Other Formats

View All FR-αβ Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-human Folate Receptors α and β (FR-αβ)	No.5/FOLR	FC
PE anti-human Folate Receptors α and β (FR-αβ)	No.5/FOLR	FC
APC anti-human Folate Receptors α and β (FR-αβ)	No.5/FOLR	FC

Certificate of Analysis

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Go To Top Version: 1 Revision Date: 01/12/2018

For Research Use Only. Not for diagnostic or therapeutic use.

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