PE anti-human HIF1alpha Antibody anti-HIF1a

Clone: 546-16 (See other available formats)
Regulatory Status: RUO
Other Names: MOP1, ARNT interacting protein, hypoxia inducible factor 1 alpha
Isotype: Mouse IgG2b, κ
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Product Citations: publications

546-16_PE_HIF1a_Antibody_FC_1_081313 — Hela cells were treated overnight with CoCl₂ (top) or left untreated (bottom), then intracellularly stained with HIF1α (clone 546-16, filled histogram) PE or mouse IgG2b, κ PE isotype control (open histogram).

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Cat #	Size	Price	Save
359703	25 tests	¥33,660
359704	100 tests	¥73,700

Description

HIF1, hypoxia induced factor 1, is a heterodimeric basic helix-loop-helix transcription factor composed of HIF1α and HIF1β. HIF1β is an aryl hydrocarbon receptor nuclear translocator (Arnt). Under normoxic conditions, HIF1α undergoes prolyl hydroxylation, which leads to degradation of HIF1α by the proteasome. Oxygen-starvation inhibits prolyl hydroxylation and prevents proteasomal degradation of HIF1α. Following nuclear translocation, HIF1α binds to the core DNA sequence 5'-[AG]CGTG-3' within the hypoxia responsive element (HRE); only the HIF1 heterodimer can bind DNA. HIF1 is a master regulator of the response to hypoxia and induces transcription of multiple genes including VEGF, glucose transporters, and proteins that aid in adaptation to hypoxic conditions. HIF1 protein is also involved in angiogenesis and energy metabolism.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant HIF1α
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
RRID: AB_2562422 (BioLegend Cat. No. 359703)
AB_2562422 (BioLegend Cat. No. 359704)

Antigen Details

Structure: 92.6 kD, contains one basic helix-loop-helix structure (bHLH), one PAS associated C - terminal (PAC) domain and two PER-ARNT-SIM (PAS) domains
Distribution: Under normoxic conditions detected in the cytoplasm; oxygen starvation causes nuclear translocation
Function: Master regulator of hypoxia in the adaptive immune response; under low oxygen conditions, induces expression of multiple genes such as VEGF, erythropoietin, and other genes that aid in adaptation to low oxygen concentrations
Interaction: Heterodimer binds 5'-[AG]CGTG-3' sequence of DNA in the hypoxia response element of target genes; interacts with COPS5, EP300, CITED2
Biology Area: Cell Biology, Immunology, Innate Immunity, Transcription Factors
Molecular Family: Nuclear Markers
Antigen References: 1. Wang GL and Semenza GL. 1993. Proc. Natl. Acad. Sci. 90:4304.
2. Wang GL and Semenza GL. 1993. J. Biol. Chem. 268:21513.
3. Wang GL and Semenza GL. 1995. J. Biol. Chem. 270:1230.
4. Galbraith MD, et al. 2013. Cell. 153:1327.
Gene ID: 3091 View all products for this Gene ID
UniProt: View information about HIF1alpha on UniProt.org

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Related FAQs

What type of PE do you use in your conjugates?: We use R-PE in our conjugates.

Other Formats

View All HIF1α Reagents Request Custom Conjugation

Description	Clone	Applications
Alexa Fluor® 647 anti-human HIF1α	546-16	ICFC
PE anti-human HIF1α	546-16	ICFC
Alexa Fluor® 488 anti-human HIF1α	546-16	ICFC

Certificate of Analysis

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Go To Top Version: 1 Revision Date: 08/15/2013

For Research Use Only. Not for diagnostic or therapeutic use.

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