PE anti-human/mouse CD3epsilon activation epitope Antibody anti-CD3e

Clone: APA1/1 (See other available formats)
Regulatory Status: RUO
Other Names: CD3e molecule, epsilon (CD3-TCR complex), CD3e antigen, CD3 epsilon
Isotype: Mouse IgG1, κ
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Product Citations: publications

Human peripheral blood lymphocytes were fixed and permeabilized with 70% ethanol, followed by intracellular staining with CD3ε (clone APA1/1) PE (filled histogram) or mouse IgG1, κ PE isotype control (open histogram).

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Cat #	Size	Price	Save
362703	25 tests	¥18,920
362704	100 tests	¥55,000

Description

The CD3ε chain of the CD3-TCR complex is a type I membrane protein that undergoes conformational change following ligation of the TCR-CD3 complex. This conformational change leads to exposure of the proline rich sequence in CD3ε and results in recruitment of Nck. It is expressed by all mature T cells and forms a heteromeric complex with CD3 α/β or γ/δ and ζ chains. It is important in T cell development, and deficiency of this gene has been associated with severe immunodeficiency.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Purified denatured CD3 proteins from human thymus
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent intracellular staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Clone APA1/1 recognizes an activation-dependent intracellular epitope of the CD3ε chain. Additional reported applications (for the relevant formats of this clone) include: immunofluorescent staining of paraformaldehyde fixed sections¹, in vivo tracking of T cells ², and Western blotting³. The LEAF purified format is recommended for in vivo studies.
RRID: AB_2564239 (BioLegend Cat. No. 362703)
AB_2564239 (BioLegend Cat. No. 362704)

Antigen Details

Structure: Type I membrane protein, part of the TCR-CD3 complex. 207 amino acids, predicted molecular weight of 23 kD.
Distribution: Mature T cells.
Function: Important role in T cell development and in linking antigen recognition with intracellular signaling pathways.
Interaction: Interacts with Nck.
Ligand/Receptor: MHC antigen.
Cell Type: T cells
Biology Area: Cell Adhesion, Cell Biology, Immunology, Signal Transduction
Molecular Family: CD Molecules, TCRs
Antigen References: 1. Savage PD, et al. 1988. Cytogenet. Cell. Genet. 49:289.
2. DeJarnette JB, et al. 1998. Proc. Natl. Acad. Sci USA. 95:14909.
3. Wang B, et al. 1999. J. Immunol. 162:88.
4. Szymczak AL, et al. 2005. J. Immunol. 175:270.
5. Borroto A, et al. 2014. J. Immunol. 192:2042.
Gene ID: 916 View all products for this Gene ID
UniProt: View information about CD3epsilon on UniProt.org

Documentation

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Related Protocols

Cell Fixation and Permeabilization Protocol using 70% Ethanol

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Description	Clone	Applications

Related Pages & Pathways

Related FAQs

What type of PE do you use in your conjugates?: We use R-PE in our conjugates.

Other Formats

View All CD3ε Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-human/mouse CD3ε (activation epitope)	APA1/1	ICFC,WB,IHC-P,in-vivo Cell Tracking,ICC,IHC,IP
PE anti-human/mouse CD3ε (activation epitope)	APA1/1	ICFC
APC anti-human/mouse CD3ε (activation epitope)	APA1/1	ICFC

Certificate of Analysis

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Go To Top Version: 1 Revision Date: 09/16/2014

For Research Use Only. Not for diagnostic or therapeutic use.

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