PE anti-human/mouse SSEA-3 Antibody anti-SSEA-3

Clone: MC-631 (See other available formats)
Regulatory Status: RUO
Other Names: Stage-Specific Embryonic Antigen-3
Isotype: Rat IgM, κ
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Product Citations: publications

NCCIT (human teratocarcinoma cell line) were stained with anti-human/mouse SSEA-3 (clone MC-631) PE (filled histogram) or rat IgM, κ PE (open histogram).

Compare all formats See PE spectral data

Cat #	Size	Price	Save
330311	25 tests	¥33,000
330312	100 tests	¥55,910

Description

Stage-Specific Embryonic Antigen-3 (SSEA-3) is an internal core structure of glob-series glycolipids (R-Galβ1-3GalNAcβ1-3Galα1-4Galβ1-R). SSEA-3 is expressed on human teratocarcinoma cells (embryonal carcinoma, EC), embryonic stem cells (ESC), embryonic germ cells (EG), and preimplantation embryos. The expression of SSEA-3 on human ESC is downregulated upon differentiation. SSEA-3 is also expressed by mouse on unfertilized eggs and preimplantation to early cleaved embryos.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Human, Mouse
Reported Reactivity: African Green
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: 4-8 cell stage mouse embryos
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunofluorescence microscopy¹.
RRID: AB_1626299 (BioLegend Cat. No. 330311)
AB_1626299 (BioLegend Cat. No. 330312)

Antigen Details

Distribution: Embryonic stemcells
Cell Type: Embryonic Stem Cells
Biology Area: Cell Biology, Immunology, Stem Cells
Gene ID: 93961 View all products for this Gene ID
UniProt: View information about SSEA-3 on UniProt.org

Documentation

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Related Pages & Pathways

Related FAQs

What type of PE do you use in your conjugates?: We use R-PE in our conjugates.

Other Formats

View All SSEA-3 Reagents Request Custom Conjugation

Description	Clone	Applications
PE anti-human/mouse SSEA-3	MC-631	FC
Alexa Fluor® 488 anti-human/mouse SSEA-3	MC-631	FC
Purified anti-human/mouse SSEA-3	MC-631	FC,ICC,ELISA
Alexa Fluor® 647 anti-human/mouse SSEA-3	MC-631	FC
APC/Cyanine7 anti-human/mouse SSEA-3	MC-631	FC
Biotin anti-human/mouse SSEA-3	MC-631	FC
FITC anti-human/mouse SSEA-3	MC-631	FC
PerCP/Cyanine5.5 anti-human/mouse SSEA-3	MC-631	FC
PE/Cyanine7 anti-human/mouse SSEA-3	MC-631	FC

Certificate of Analysis

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Go To Top Version: 2 Revision Date: 07/08/2013

For Research Use Only. Not for diagnostic or therapeutic use.

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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