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PE anti-mouse CD122 (IL-2Rβ) Antibody

Pricing & Availability
Clone
5H4 (See other available formats)
Regulatory Status
RUO
Other Names
IL-2 Receptor β chain, IL-2Rβ
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
5H4_PE_061609.jpg
C57BL/6 mouse splenocytes stained with 5H4 PE and CD3 APC
  • 5H4_PE_061609.jpg
    C57BL/6 mouse splenocytes stained with 5H4 PE and CD3 APC
Compare all formats See PE spectral data
Cat # Size Price Save
105905 25 µg ¥15,310
105906 100 µg ¥38,100
Description

CD122 is a 70-75 kD IL-2 receptor β chain also known as IL-2Rβ, which is also shared by the IL-15 receptor. It is constitutively expressed by NK cells and at lower levels by T cells, B cells, monocytes, and macrophages. The IL-2Rβ chain can combine with either the common γ subunit (γc, CD132) alone or with the γc subunit and the IL-2Rα subunit (CD25) to generate intermediate or high affinity IL-2 receptor complexes, respectively. CD122 expression levels can be upregulated by activation. The 5H4 antibody does not block IL-2 binding to the IL-2 receptor. CD122 is expressed on murine, but not human, CD8+ Tregs involved in the maintenance of T cell homeostasis.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Rat myeloma YB2/0 transfected with truncated mouse Il-2Rβ cDNA
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1,2.

Application References

(PubMed link indicates BioLegend citation)
  1. Furse R, et al. 1993 Eur. J. Immunol. 23:3181. (IP)
  2. He YW, et al. 1995. J. Immunol. 154:1596. (IP)
  3. Liqons DL, et al. 2012. J Biol Chem. 287:34386. PubMed.
Product Citations
  1. Misumi I et al. 2019. Cell Rep. 27(2):514-524 . PubMed
  2. Sekiya T et al. 2018. Cell reports. 24(6):1627-1638 . PubMed
  3. Dikiy S, et al. 2021. Immunity. 54(5):931-946.e11. PubMed
  4. Snell LM, et al. 2018. Immunity. 49:678. PubMed
  5. Amezcua Vesely MC, et al. 2020. Cell. 178(5):1176-1188.e15.. PubMed
RRID
AB_2125736 (BioLegend Cat. No. 105905)
AB_2125736 (BioLegend Cat. No. 105906)

Antigen Details

Structure
Ig superfamily, forms high affinity IL-2 receptor with CD25 and CD132 chains or intermediate affinity receptor with CD132 alone, 70-75 kD
Distribution

T cells and B cells, NK cells, monocytes, macrophages

Function
Critical component of IL-2 and IL-15 signaling
Ligand/Receptor
IL-2, IL-15
Cell Type
B cells, Macrophages, Monocytes, NK cells, T cells, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Minami Y, et al. 1993. Annu. Rev. Immunol. 11:245.
3. Suzuki H, et al. 1995. Science 268:1472.
4. Shi Z, et al. 2009. Eur. J. Immunol. 39:2109.

Gene ID
16185 View all products for this Gene ID
UniProt
View information about CD122 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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