Loading...

PE anti-mouse CD223 (LAG-3) Antibody

Pricing & Availability
Clone
C9B7W (See other available formats)
Regulatory Status
RUO
Other Names
LAG3, LAG-3
Isotype
Rat IgG1, κ
Ave. Rating
Submit a Review
Product Citations
publications
C9B7W_PE_060308
ConA-stimulated C57BL/6 splenocytes stained with C9B7W PE
  • C9B7W_PE_060308
    ConA-stimulated C57BL/6 splenocytes stained with C9B7W PE
Compare all formats See PE spectral data
Cat # Size Price Save
125207 25 µg ¥15,670
125208 200 µg ¥41,910
Description

CD223, also known as LAG-3, is 70 kD type I transmembrane glycoprotein within the Ig superfamily. It is the gene product of lymphocyte activation gene-3 (Lag3) with structural similarity to CD4. CD223 is expressed on activated T cells and NK cells, as well as T regulatory cells. CD223, like CD4, binds MHC class II molecules, but with higher affinity than CD4. In association with the CD3 complex, CD223 has been shown as a negative regulatory molecule to inhibit TCR-mediated signaling and regulate homeostatic T cell expansion. In contrast to inhibition, CD223 has an activation function to promote immune responses by inducing MHC class II signaling in antigen presenting cells.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Murine CD223-Ig fusion protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Workman CJ, et al. 2002. Eur. Immunol. 32:2255. (IP, ELISA)
  2. Workman CJ, et al. 2005. J. Immunol. 174:688. (FC)
  3. Li N, et al. 2004. J. Immunol. 173:6806. (IP, ELISA)
Product Citations
  1. Balood M, et al. 2022. Nature. 611:405. PubMed
  2. Wei M, et al. 2022. Front Immunol. 13:1017574. PubMed
  3. Cheng H, et al. 2023. Nat Metab. 5:314. PubMed
  4. Kemna J, et al. 2023. Nat Immunol. 24:414. PubMed
  5. Bertrand F, et al. 2017. Nat Commun. 8:2256. PubMed
  6. Logan K Smith et al. 2018. Immunity. 48(2):299-312 . PubMed
  7. Dong MB, et al. 2020. Cell. 178(5):1189-1204.e23.. PubMed
  8. Swan SL, et al. 2023. Front Immunol. 14:1085547. PubMed
  9. Pandit M, et al. 2023. Nat Commun. 14:2593. PubMed
  10. Tian H, et al. 2022. Cancer Sci. 113:875. PubMed
  11. Clement M, et al. 2016. PLoS Pathog. 12:e1006050. PubMed
  12. Lu SX, et al. 2021. Cell. . PubMed
  13. Yu AI, et al. 2020. Cell Rep. 107471:31. PubMed
  14. Rodriguez AB, et al. 2021. Cell Reports. 36(3):109422. PubMed
  15. Mitchell JE, et al. 2021. Cell Reports. 35(2):108966. PubMed
  16. Wagle MV, et al. 2021. Nat Commun. 12:2782. PubMed
  17. Lv Z, et al. 2020. Oncoimmunology. 9:1747688. PubMed
  18. Gozgit JM, et al. 2021. Cancer Cell. :. PubMed
  19. Hu Q, et al. 2018. Nat Biomed Eng. 0.660416667. PubMed
  20. Zuo S, et al. 2021. J Immunother Cancer. 9:. PubMed
  21. Fortner K, et al. 2017. Journal of Autoimmunity. 10.1016/j.jaut.2017.05.003. PubMed
  22. Shen M, et al. 2022. Nat Cancer. 3:60. PubMed
  23. RY H, et al. 2016. Oncoimmunology. 6:e1249561. PubMed
  24. Yang BH, et al. 2020. Cell Reports. 27(12):3629-3645.e6.. PubMed
  25. Wedekind MF, et al. 2021. iScience. 24(7):102759. PubMed
  26. Alenazy MF, et al. 2022. PLoS One. 17:e0271689. PubMed
  27. De Souza ALS, et al. 2018. Neurol Ther. 7:103. PubMed
  28. Lu Y, et al. 2018. Cancer Cell. 33:1048. PubMed
  29. Katsuyama T, et al. 2021. Cell Reports. 36(1):109339. PubMed
  30. Rudd CE, et al. 2020. Cell Reports. 30(7):2075-2082. PubMed
RRID
AB_2133343 (BioLegend Cat. No. 125207)
AB_2133343 (BioLegend Cat. No. 125208)

Antigen Details

Structure
Ig superfamily
Distribution

T cells and NK cells, as well as T regulatory cells

Cell Type
NK cells, T cells, Tregs
Biology Area
Immunology, Inhibitory Molecules
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Baixeras E et al. 1992. J. Exp. Med. 176:327.

Gene ID
16768 View all products for this Gene ID
UniProt
View information about CD223 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account