- Clone
- F011-5 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- LRRC32, Garpin, Glycoprotein A repetitions predominant
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![F011-5_PE_1_112811 F011-5_PE_1_112811](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/F011-5_PE_1_112811.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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CD3+CD28+IL-2-stimulated C57BL/6 mouse splenocytes (48 hours) were surface stained with CD4 FITC, and GARP (clone F011-5) PE (top) or rat IgG2a, κ PE isotype control (bottom), then intracellularly stained with FOXP3 Alexa Fluor® 647. Data shown was generated by gating on the CD4+ lymphocyte population. -
Cat # | Size | Price | Save |
---|---|---|---|
142903 | 25 µg | ¥34,980 | |
142904 | 100 µg | ¥80,300 |
Glycoprotein A Repetitions Predominant (GARP), also known as leucine rich repeat containing protein 32 (LRC32), is a 80 kD type I membrane glycoprotein with 20 leucine rich repeats in the extracellular portion of the protein. GARP was found on the surface of megakaryocytes, platelets, and activated Tregs. It serves as a receptor for latent TGF-β. Recent evidence suggests that GARP may play a role in controlling suppressor function of Tregs. A mutation in GARP has been reported in a large Samaritan kindred with Usher syndrome type 1. In addition, it has been found that GARP mRNA is highly amplified in different tumors, which indicates that tumor cells may use GARP to express TGF-β or to capture TGF-β from their surroundings, resulting in local suppression of anti-tumor immune responses or the induction of Tregs.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Mouse GARP (extracellular domain)
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
-
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Product Citations
-
- RRID
-
AB_10983065 (BioLegend Cat. No. 142903)
AB_10962944 (BioLegend Cat. No. 142904)
Antigen Details
- Structure
- 80 kD transmembrane GARP glycoprotein with an extracellular region containing 20 leucine-rich repeats
- Distribution
- Activate Tregs, megakaryocytes, platelets
- Ligand/Receptor
- LAP (TGF-β1)
- Cell Type
- Tregs, Megakaryocytes, Platelets
- Biology Area
- Cell Biology, Immunology, Signal Transduction
- Antigen References
-
1. Ollendorff V, et al. 1994. Cell. Growth Differ. 5:213.
2. Stockis J, et al. 2009. Eur. J. Immunol. 39:3315.
3. Wang R, et al. 2009. P. Natl. Acad. Sci. USA 106:13439.
4. Tran DQ, et al. 2009. P. Natl. Acad. Sci. USA 106:13445. - Gene ID
- 434215 View all products for this Gene ID
- UniProt
- View information about GARP on UniProt.org
Related FAQs
- What type of PE do you use in your conjugates?
- We use R-PE in our conjugates.
Other Formats
View All GARP Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Alexa Fluor® 647 anti-mouse GARP (LRRC32) | F011-5 | FC |
Purified anti-mouse GARP (LRRC32) | F011-5 | FC |
PE anti-mouse GARP (LRRC32) | F011-5 | FC |
PE/Cyanine7 anti-mouse GARP (LRRC32) | F011-5 | FC |
APC/Fire™ 750 anti-mouse GARP (LRRC32) | F011-5 | FC |
PE/Dazzle™ 594 anti-mouse GARP (LRRC32) | F011-5 | FC |
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Alexa Fluor® 647 anti-mouse GARP (LRRC32)
CD3+CD28+IL-2-stimulated (48 hours) C57BL/6 mouse splenocyte... -
Purified anti-mouse GARP (LRRC32)
C57BL/6 mouse platelets were stained with purified GARP (clo... -
PE anti-mouse GARP (LRRC32)
CD3+CD28+IL-2-stimulated C57BL/6 mouse splenocytes (48 hours... -
PE/Cyanine7 anti-mouse GARP (LRRC32)
Balb/c mouse platelets were stained with stained with True-S... -
APC/Fire™ 750 anti-mouse GARP (LRRC32)
CD3+CD28+IL-2-stimulated (48 hours) C57BL/6 mouse splenocyte... -
PE/Dazzle™ 594 anti-mouse GARP (LRRC32)
CD3+CD28+IL-2-stimulated (48 hours) C57BL/6 mouse splenocyte...
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