PE anti-mouse/human SEMA4A Antibody anti-SEMA4A

Clone: 5E3/SEMA4A (See other available formats)
Regulatory Status: RUO
Other Names: Semaphorin 4A, SEMAB, Sema B, Semaphorin B, RP35, CORD10, SEMA 4A
Isotype: Mouse IgG1, λ
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Product Citations: publications

5E3-SEMA4A_PE_SEMA4A_Antibody_FC_1_010815 — Mouse splenocytes were stained with CD3 APC and Semaphorin 4A (clone 5E3/SEMA4A) PE (top) or mouse IgG1 PE isotype control (bottom).

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Cat #	Size	Price	Save
148403	25 µg	¥28,380
148404	100 µg	¥64,240

Description

Semaphorin 4A (SEMA4A) is a class IV semaphorin of soluble and transmembrane proteins originally identified as SemB. It is a single-pass type I membrane protein containing an immunoglobulin-like C2-type domain, a PSI domain, and a Sema domain. SEMA4A is expressed on dendritic cells, activated B cells, and T cells. It is also found on different tissues, such as those in brain, lung, kindney, spleen, and testis. In the immune system, SEMA4A functions as an activator in the T-cell-mediated immune response and suppresses vascular endothelial growth factor (VEGF)-mediated endothelial cell migration and proliferation in vitro and angiogenesis in vivo. Like other semaphorin family members it is also involved in guidance of axonal migration during neuronal development and in immune responses.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Mouse, Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Mouse SEMA4A-Fc proteins
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Additional reported applications (for relevant formats) include: ELISA¹.
RRID: AB_2565286 (BioLegend Cat. No. 148403)
AB_2565286 (BioLegend Cat. No. 148404)

Antigen Details

Structure: Single-pass type I membrane protein containing an immunoglobulin-like C2-type domain, a PSI domain and a SEMA domain.
Distribution: Dendritic cells, T cells, B cells, brain, lung, kindney, spleen and testis
Function: An activator in T-cell-mediated immune response. Suppresses vascular endothelial growth factor (VEGF)-mediated endothelial cell migration and proliferation in vitro and angiogenesis in vivo. It is also involved in guidance of axonal migration during neuronal development.
Cell Type: Dendritic cells, T cells, B cells, Tregs
Biology Area: Cell Biology, Immunology, Innate Immunity, Neuroscience, Synaptic Biology
Molecular Family: Adhesion Molecules
Antigen References: 1. Kumanogoh A, et al. 2002. Nature. 419:629.
2. Kumanogoh A, et al. 2005. Immunity. 22:305.
3. Toyofuku T, et al. 2007. EMBO J. 26:1373.
4. Delgoffe GM, et al. 2013. Nature. 501:252.
Gene ID: 20351 View all products for this Gene ID 64218 View all products for this Gene ID
UniProt: View information about SEMA4A on UniProt.org

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Cell Surface Flow Cytometry Staining Protocol

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Description	Clone	Applications

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Related FAQs

What type of PE do you use in your conjugates?: We use R-PE in our conjugates.

Other Formats

View All SEMA4A Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-mouse/human SEMA4A	5E3/SEMA4A	FC,ELISA
PE anti-mouse/human SEMA4A	5E3/SEMA4A	FC
APC anti-mouse/human SEMA4A	5E3/SEMA4A	FC

Certificate of Analysis

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Go To Top Version: 1 Revision Date: 01/21/2015

For Research Use Only. Not for diagnostic or therapeutic use.

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