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PE anti-mouse PD-1H (VISTA) Antibody

Pricing & Availability
Clone
A17207C (See other available formats)
Regulatory Status
RUO
Other Names
PD-1 homolog, Dies1, B7-H5
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
A17207C_PE_PD-1H_Antibody_021920
C57BL/6 mouse splenocytes were stained with CD8a APC and anti-mouse PD-1H (VISTA) (clone A17207C) (left) PE or rat IgG 2a, κ isotype control (right) PE.
  • A17207C_PE_PD-1H_Antibody_021920
    C57BL/6 mouse splenocytes were stained with CD8a APC and anti-mouse PD-1H (VISTA) (clone A17207C) (left) PE or rat IgG 2a, κ isotype control (right) PE.
See PE spectral data
Cat # Size Price Save
159503 25 µg ¥33,660
159504 100 µg ¥82,940
Description

PD-1H, also known as VISTA, is a 309 aa type I transmembrane protein, composed of seven exons. PD-1H has one Ig-V like domain, and its sequence is similar to the Ig-V domains of the members of CD28 and B7 families. PD-1H is expressed by a subset of T cells, macrophages, dendritic cells, neutrophils, and NK cells. It has been proposed that PD-1H can be useful to modulate the host immune response to allogeneic transplants. 

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant mouse VISTA protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
0.2 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Clone A17207C antibody does not affect the binding of clone MIH63 nor clone MH5A.

RRID
AB_2890722 (BioLegend Cat. No. 159503)
AB_2890722 (BioLegend Cat. No. 159504)

Antigen Details

Structure
Type I transmembrane protein, member of the CD28 family
Distribution

Macrophages, dendritic cells, neutrophils, NK cells, naïve and activated T cells

Function
Negative regulation of immune response
Ligand/Receptor
PSGL-1
Cell Type
Dendritic cells, Macrophages, Neutrophils, NK cells, T cells, Tregs
Biology Area
Immunology, Inhibitory Molecules
Molecular Family
Soluble Receptors
Antigen References
  1. Flies DB, et al. 2011. J. Immunol. 187:1537.
  2. Wang Li, et al. 2011. J. Exp Med. 208:577.
Gene ID
74048 View all products for this Gene ID
UniProt
View information about PD-1H on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 02/19/2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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