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PE anti-mouse/rat/human CD27 Antibody

Pricing & Availability
Clone
LG.3A10 (See other available formats)
Regulatory Status
RUO
Other Names
T14, S152, Tp55, TNFRSF7
Isotype
Armenian Hamster IgG
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Product Citations
publications
a-LGdot3A10_PE_021808
C57BL/6 mouse splenocytes stained with LG.3A10 PE
  • a-LGdot3A10_PE_021808
    C57BL/6 mouse splenocytes stained with LG.3A10 PE
  • b-LGdot3A10_PE_CD27_Antibody_2_101024
    Multiplexed IHC staining of PE anti-CD27 (clone LG.3A10) on formalin-fixed paraffin-embedded human tonsil tissue, validated for use on the Cellscape™. The tissue was iteratively stained with PE anti-CD27 (clone LG.3A10, green) and Alexa Fluor® 488 anti-CD3 (red) for one hour at room temperature. Nuclei were counterstained with Hoechst 33342. Images were captured with a 20X objective. Scale bar: 50 µm
Compare all formats See PE spectral data
Cat # Size Price Save
124209 50 µg ¥20,830
124210 200 µg ¥59,630
Description

CD27 is also known as S152 and T14. A member of the tumor necrosis factor receptor (TNFR) superfamily, it is a 45 kD protein expressed on peripheral T cells, memory B cells, NK cells, and thymocyte subset. Through its ligand, CD70, CD27 plays a key role in T cell and B cell interactions. Additionally, ligation of CD27 on naïve T cells may be important in their maturation to effector cells.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse, Rat, Human
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
Armenian hamster fibroblast line ARHO12 transfected with mouse CD27 cDNA
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
SB - Community Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: functional assay1,3 and immunohistomecial staining of acetone-fixed frozen sections2. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 124243 & 124244).

Additional Product Notes

For the use of this antibody in spatial biology (SB), we have partnered with Bruker Spatial Biology Biosciences for demonstration of this antibody on their next-generation ChipCytometry instrument called the CellScape™. The CellScape platform is an end-to-end solution for highly multiplexed spatial omics. Combining an advanced, purpose-built imaging system with easy-to-use fluidics for walk-away automation, the CellScape system will accelerate your exploration into the rapidly evolving field of spatial biology. More information on the the Bruker Spatial Biology CellScape and a complete list of our antibodies that have been demonstrated on the instrument can be found here.

Application References

(PubMed link indicates BioLegend citation)
  1. Gravestein LA, et al. 1995. Int. Immunol. 7:551. (FA)
  2. Gravestein LA, et al. 1996. J. Exp. Med. 184:675. (IHC)
  3. Takeda K, et al. 2000. J. Immunol. 164:1741. (FA)
  4. Welner RS, et al. 2009. J. Immunol. 183:7768. PubMed
  5. Vicetti Miguel RC, et al. 2012. J. Immunol. 189:3449. PubMed
  6. White CA, et al. 2014. J Immunol. 193:5933. PubMed
  7. Iwata S, et al. 2015. Lupus. 24:695. PubMed
Product Citations
  1. Liu L, et al. 2022. Nat Commun. 13:6740. PubMed
  2. Wu R, et al. 2022. Nat Immunol. 23:1536. PubMed
  3. Dong MB, et al. 2020. Cell. 178(5):1189-1204.e23.. PubMed
  4. Fu B et al. 2017. Immunity. 47(6):1100-1113 . PubMed
  5. Liu X, et al. 2021. eLife. 0.416666666666667. PubMed
  6. Beatson RE, et al. 2021. Cell Rep Med. 2:100473. PubMed
  7. Xiong Y, et al. 2018. Int J Clin Exp Pathol. 3.803472222. PubMed
  8. Cong J et al. 2018. Cell metabolism. 28(2):243-255 . PubMed
  9. Tacconi C, et al. 2021. Cell Reports. 35(2):108993. PubMed
  10. Ni J, et al. 2020. Immunity. 52(6):1075-1087.e8. PubMed
  11. Shibata K, et al. 2011. Blood. 118:586. PubMed
  12. Yu X, et al. 2019. Nat Commun. 10:574. PubMed
  13. NULL, et al. 2022. Cell. 185:916. PubMed
  14. Wagner AK, et al. 2022. iScience. 25:105137. PubMed
  15. Xiong Y, et al. 2019. BMC Immunol. 20:24. PubMed
  16. Wang J, et al. 2020. Cell. 183(7):1867-1883.e26. PubMed
  17. Kujur W, et al. 2020. PLoS Pathog. 16:e1009132. PubMed
RRID
AB_1236459 (BioLegend Cat. No. 124209)
AB_1236459 (BioLegend Cat. No. 124210)

Antigen Details

Structure
TNFR superfamily, 45 kD
Distribution

Peripheral blood T cells, memory B cells, NK cells, thymocyte subset

Function
Costimulatory signal for T and B cell activation, T cell development
Interaction
TRAF2, TRAF5, CD27-binding protein (Siva)
Ligand/Receptor
CD70
Cell Type
B cells, NK cells, T cells, Thymocytes
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Hintzen RQ, et al. 1994. Immunol. Today 15:307.
2. Akiba H, et al. 1998. J. Biol. Chem. 273:13353.
3. Kobata T, et al. 1995. P. Natl. Acad. Sci. USA 92:11249.
4. Hendriks J, et al. 2000. Nat. Immunol. 1:433
5. Hintzen RQ, et al. 1994. Int. Immunol. 6:477
6. Camerini D, et al. 1991. J. Immunol. 147:3165

Gene ID
21940 View all products for this Gene ID 939 View all products for this Gene ID 500318 View all products for this Gene ID
UniProt
View information about CD27 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
If an antibody clone has been previously successfully used in IBEX in one fluorescent format, will other antibody formats work as well?

It’s likely that other fluorophore conjugates to the same antibody clone will also be compatible with IBEX using the same sample fixation procedure. Ultimately a directly conjugated antibody’s utility in fluorescent imaging and IBEX may be specific to the sample and microscope being used in the experiment. Some antibody clone conjugates may perform better than others due to performance differences in non-specific binding, fluorophore brightness, and other biochemical properties unique to that conjugate.

Will antibodies my lab is already using for fluorescent or chromogenic IHC work in IBEX?

Fundamentally, IBEX as a technique that works much in the same way as single antibody panels or single marker IF/IHC. If you’re already successfully using an antibody clone on a sample of interest, it is likely that clone will have utility in IBEX. It is expected some optimization and testing of different antibody fluorophore conjugates will be required to find a suitable format; however, legacy microscopy techniques like chromogenic IHC on fixed or frozen tissue is an excellent place to start looking for useful antibodies.

Are other fluorophores compatible with IBEX?

Over 18 fluorescent formats have been screened for use in IBEX, however, it is likely that other fluorophores are able to be rapidly bleached in IBEX. If a fluorophore format is already suitable for your imaging platform it can be tested for compatibility in IBEX.

The same antibody works in one tissue type but not another. What is happening?

Differences in tissue properties may impact both the ability of an antibody to bind its target specifically and impact the ability of a specific fluorophore conjugate to overcome the background fluorescent signal in a given tissue. Secondary stains, as well as testing multiple fluorescent conjugates of the same clone, may help to troubleshoot challenging targets or tissues. Using a reference control tissue may also give confidence in the specificity of your staining.

How can I be sure the staining I’m seeing in my tissue is real?

In general, best practices for validating an antibody in traditional chromogenic or fluorescent IHC are applicable to IBEX. Please reference the Nature Methods review on antibody based multiplexed imaging for resources on validating antibodies for IBEX.

Go To Top Version: 3    Revision Date: 10/10/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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