- Clone
- A21012B (See other available formats)
- Regulatory Status
- RUO
- Other Names
- NF-kB p65, RelA,P65, NFKB3, Nuclear Factor NF-Kappa-B P65 subunit, Transcription Factor P65, NF-Kappa-B Transcription Factor P65, RELA Proto-Oncogene NF-KB subunit
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Save |
---|---|---|---|
614153 | 25 tests | ¥33,000 | |
614154 | 100 tests | ¥83,600 |
NF-κB p65, also known as NFKB3 and RelA, is one of five members of the nuclear factor κB (NF-κB)/Rel family. Inactive NF-κB p65 is sequestered in the cytoplasm as a complex with IκB inhibitory proteins. Upon activation of the NF-κB pathway, IκB proteins are degraded, thus releasing NF-κB p65 to the nucleus. Binding of NF-κB p65 to transcriptional cofactors is induced via phosphorylation on serines 276, 529, 536, and 471. As a transcription factor, NF-κB p65 is vital to cell proliferation, immune response, survival, and apoptosis. Through regulation of chondrocyte and osteoblast differentiation and survival, NFκB p65 has a crucial role in skeletal development. Overexpressed in several cancers, NF-κB p65 functions as an oncogene and has been implicated in tumorigenesis, metastasis, tumor angiogenesis, and chemoresistance.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Synthetic peptide corresponding to human NF-κB p65 phosphorylated at serine 529
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
ICFC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Phos™ Perm Buffer in Cell Suspensions Protocol. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
-
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
-
When testing by ICC, we recommend fixation with 4% PFA followed by permeabilization with Triton X-100.
- RRID
-
AB_3097548 (BioLegend Cat. No. 614153)
AB_3097548 (BioLegend Cat. No. 614154)
Antigen Details
- Structure
- NF-κB p65 is a 551 amino acid protein with a predicted molecular weight of 60 kD.
- Distribution
-
Ubiquitously expressed/ Cytoplasm and Nucleus
- Function
- Transcription, RAS pathway, host-virus interaction, oncogene
- Interaction
- NF-kappa-B, TLE5, TLE1, TP53BP2, GSK3B, MAP3K14, HDAC3, AKIP1, FOXP3
- Biology Area
- Cell Biology, Immuno-Oncology, Immunology, Innate Immunity, Transcription Factors
- Molecular Family
- Innate Immune Signaling, Phospho-Proteins, Protein Kinases/Phosphatase
- Antigen References
-
- Giridharan S and Srinivasan M. 2018. J Inflamm Res. 11:407-419.
- Ren C, et al. 2022. Cell Death Differ. 29:381-392.
- Yu S, et al. 2020. FEBS Lett. 594:13681378.
- Gene ID
- 5970 View all products for this Gene ID
- UniProt
- View information about NF-kappaB p65 on UniProt.org
Related FAQs
- What type of PE do you use in your conjugates?
- We use R-PE in our conjugates.
Other Formats
View All NF-κB p65 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-NF-κB p65 Phospho (Ser529) | A21012B | WB,ICC,ICFC |
PE anti-NF-κB p65 Phospho (Ser529) | A21012B | ICFC |
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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Purified anti-NF-κB p65 Phospho (Ser529)
HeLa cells were fixed with 4% PFA Fixation Buffer (Cat. No. ... Whole cell extracts (15 µg total protein) from untreated HeL... HeLa cells treated with 20 ng/ml TNFα and 100 nM Calyculin A... -
PE anti-NF-κB p65 Phospho (Ser529)
Peripheral blood mononuclear cells untreated (negative contr...
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