PE anti-p53 Antibody anti-p53

Clone: DO-7 (See other available formats)
Regulatory Status: RUO
Other Names: Tumor protein p53 (TP53)
Isotype: Mouse IgG2b, κ
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Product Citations: publications

DO-7_PE_p53_Antibody_FC_062513 — A431 cells, which are a human epidermoid carcinoma cell line, was fixed and permeabilized with FOXP3 Fix/Perm Buffer Set, and then stained with p53 (clone DO-7) PE (filled histogram) or mouse IgG2b, κ PE isotype control (open histogram).

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Cat #	Size	Price	Save
645805	25 tests	¥32,120
645806	100 tests	¥71,060

Description

p53 is a 53 kD protein that forms tetramers and functions as a tumor suppressor and transcriptional activator of genes that inhibit growth and/or invasion, cell cycle checkpoint after irradiation, DNA repair, apoptotic induction, signal transduction, and cell adhesion. This protein is localized to the nucleus when activated and can be upregulated by genotoxic or other cellular stresses. p53 is modified by phosphorylation, acetylation, ribosylation, ubiquitination, and sumoylation; ubiquination targets p53 for degradation via mdm2. This protein interacts with a variety of proteins including mdm2, mdmx, topoisomerase I, PML3, Bcl-XL, Bcl-2, Chk1, JNK, p38, HIPK2, CK2, DNA-PK, p300/CBP, PCAF, PARP1, and HDAC1-3. Mutant p53 associates with p63 and p73.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: p53 N-terminal linear epitope aa 20-25
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
RRID: AB_2562267 (BioLegend Cat. No. 645805)
AB_2562267 (BioLegend Cat. No. 645806)

Antigen Details

Structure: Tetramer; 53 kD
Distribution: Nuclear when activated
Function: Tumor suppressor, transcription factor regulates cell cycle checkpoint, apoptosis, DNA integrity
Interaction: mdm2, mdmx, TopoI, PML3, Bcl-X_L, Bcl-2, Chk1, JNK, p38, HIPK2, CK2, DNA-PK, p300/CBP, PCAF, PARP1, HDAC1-3; mutant p53 associates with p63, p73
Cell Type: Mesenchymal Stem Cells
Biology Area: Cell Biology, DNA Repair/Replication, Stem Cells
Gene ID: 7157 View all products for this Gene ID
UniProt: View information about p53 on UniProt.org

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Description	Clone	Applications

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Pathways

Related FAQs

What type of PE do you use in your conjugates?: We use R-PE in our conjugates.

Other Formats

View All p53 Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-p53	DO-7	WB,ICC,ChIP,ICFC,ELISA
FITC anti-p53	DO-7	ICFC
PE anti-p53	DO-7	ICFC

Certificate of Analysis

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Go To Top Version: 2 Revision Date: 06/14/2022

For Research Use Only. Not for diagnostic or therapeutic use.

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