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- Clone
- OX-22 (See other available formats)
- Regulatory Status
- RUO
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
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LOU rat splenocytes stained with OX-22 PE
CD45RC is a 200-220 kD single chain type I membrane glycoprotein. It is an exon 5 splice variant (exon C) of the tyrosine phosphatase CD45. The CD45RC isoform is expressed on B cells, majority of CD8+ T cells and a subset of CD4+ T cells, but not on myeloid cells. CD45 and its isoforms non-covalently associate with lymphocyte phosphatase-associated phosphoprotein (LPAP) on T and B lymphocytes. CD45 has been reported to be associated with several other cell surface antigens, including CD1, CD2, CD3, and CD4. CD45 is involved in signaling lymphocytes activation.
Product DetailsProduct Details
- Verified Reactivity
- Rat
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- PHA-activated rat lymph node cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
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FC - Quality tested
- Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.06 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
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Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
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Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections2, 3 and formalin-fixed paraffin-embedded tissue sections.
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Application References
(PubMed link indicates BioLegend citation) -
- Woollett GR, et al. Eur. J. Immunol. 15:168.
- Spickett GP, et al. 1983. J. Exp. Med. 158:795.
- Mason DW, et al. 1983. Immunol. Rev. 74:57.
- Product Citations
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- RRID
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AB_1186138 (BioLegend Cat. No. 204007)
Antigen Details
- Cell Type
- B cells
- Biology Area
- Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- CD Molecules
- Gene ID
- 544392 View all products for this Gene ID 544377 View all products for this Gene ID
- UniProt
- View information about CD45RC on UniProt.org
Related FAQs
- What type of PE do you use in your conjugates?
- We use R-PE in our conjugates.
Other Formats
View All CD45RC Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| PE anti-rat CD45RC | OX-22 | FC |
| Alexa Fluor® 647 anti-rat CD45RC | OX-22 | FC,IHC-F |
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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PE anti-rat CD45RC
LOU rat splenocytes stained with OX-22 PE -
Alexa Fluor® 647 anti-rat CD45RC
LOU rat splenocytes stained with ox-22 Alexa Fluor® 647 
Rat frozen spleen section was fixed with 4% paraformaldehyde...
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