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PE anti-rat TSPAN8 Antibody

Pricing & Availability
Clone
2D9/TSPAN8 (See other available formats)
Regulatory Status
RUO
Other Names
Tspan8, tetraspanin-8
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
2D9_PE_TSPAN8_Antibody_021323
Rat colorectal cancer cell line ACL-15 stained with anti-rat TSPAN8 (clone 2D9/TSPAN8) PE (filled histogram) or mouse IgG2a, κ PE isotype control (open histogram).
  • 2D9_PE_TSPAN8_Antibody_021323
    Rat colorectal cancer cell line ACL-15 stained with anti-rat TSPAN8 (clone 2D9/TSPAN8) PE (filled histogram) or mouse IgG2a, κ PE isotype control (open histogram).
See PE spectral data
Cat # Size Price Save
200803 25 µg ¥33,440
200804 100 µg ¥75,900
Description

Tetraspanins, also referred to as the transmembrane 4 superfamily (TM4SF) proteins, have four transmembrane alpha-helices and two extracellular domains. It acts as molecular facilitators forming a web between themselves and a multitude of molecules. It is also involved in adhesion, motility, matrix modulation and cell fusion. Tetraspanins/Tetraspanin-complexes are constitutive components of exosomes. During exosome assembly, Tetraspanins become internalized via a tyrosine-based internalization motif. Internalized membrane microdomains are recruited into early endosomes from where they can become incorporated into multivesicular body (MVB), which migrate towards and fuse with the cell membrane releasing invaginated endosomes as exosomes.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Rat
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
0.2 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
RRID
AB_2936740 (BioLegend Cat. No. 200803)
AB_2936740 (BioLegend Cat. No. 200804)

Antigen Details

Structure
Four transmembrane alpha-helices and two extracellular domains
Distribution

Multi-pass membrane protein

Function
Adhesion, motility, matrix modulation and cell fusion
Interaction
LIPR1, MEP1B, STX1-2, TIM23, S38A7
Ligand/Receptor
Integrin binding
Biology Area
Immunology
Antigen References
  1. Berditchevsk F, et al. 2001. Journal of Cell Science. 114 (Pt 23): 4143–51.
  2. Mu W, et al. 2020. Cells. 9:319.
Gene ID
171048 View all products for this Gene ID
UniProt
View information about TSPAN8 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 02/16/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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