PE anti-Syk Antibody anti-Syk

Clone: 4D10.2 (See other available formats)
Regulatory Status: RUO
Other Names: Spleen tyrosine kinase, protein tyrosine kinase SYK
Isotype: Mouse IgG2a, κ
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Product Citations: publications

4D102_PE_Syk_Antibody_1_081018 — Human peripheral blood lymphocytes were surface stained with CD3 Brilliant Violet 421™ then treated with the True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) and intracellularly stained with anti-Syk (clone 4D10.2) PE (left) or mouse IgG2a, κ PE isotype control (right)

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Cat #	Size	Price	Save
644303	25 tests	¥31,020
644304	100 tests	¥76,340

Description

Syk is a cytoplasmic 72 kD non-receptor tyrosine kinase that is widely expressed in hematopoietic cells. The Syk kinase associates with a number of proteins including c-Src, Lck, Lyn, Fyn, Vav1, STAT3, SHP1, Grb2, and LAT to couple immunoreceptors to downstream signaling events mediating cellular proliferation, differentiation, and phagocytosis. Syk is modified by phosphorylation on multiple tyrosine sites

Product Details

Technical data sheet

Product Details

Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human Syk peptide aa 314-339
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: This product is for in vitro research use only. It is not to be used for commercial purposes. Use of this product to produce products for sale or for diagnostic therapeutic or drug discovery purposes is prohibited. In order to obtain a license to use this product for commercial purposes, contact The Regents of the University of California.

Intracellular stained with Nuclear Factor Fixation and Permeabilization Buffer Set for Flow Cytometry.
RRID: AB_2565303 (BioLegend Cat. No. 644303)
AB_2565303 (BioLegend Cat. No. 644304)

Antigen Details

Structure: Non-receptor associated tyrosine kinase, contains 2 SH2 domains, 72 kD
Distribution: Cytoplasmic protein, widely expressed in hematopoietic cells
Function: Couples immunoreceptor to downstream signaling events that mediate cellular responses including proliferation, differentiation, and phagocytosis
Biology Area: Cell Biology, Immunology, Signal Transduction
Antigen References: 1. Taniguchi T, et al. 1991. J. Biol. Chem. 266:15790.
2. Toyabe SL, et al. 2001.Immunology 103:164.
Gene ID: 6850 View all products for this Gene ID
UniProt: View information about Syk on UniProt.org

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Pathways

Related FAQs

What type of PE do you use in your conjugates?: We use R-PE in our conjugates.

Other Formats

View All Syk Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-Syk	4D10.2	WB,IP,ICFC,IHC-P
PE anti-Syk	4D10.2	ICFC
APC anti-Syk	4D10.2	ICFC

Certificate of Analysis

Go To Top Version: 1 Revision Date: 02/03/2015

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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