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PE/Cyanine7 anti-human ZAP70 Phospho (Tyr493) Antibody

Pricing & Availability
Clone
A16043E (See other available formats)
Regulatory Status
RUO
Other Names
SRK, STD, TZK, p Zeta-Chain (TCR) Associated Protein Kinase 70 kD, p 70 KD Zeta-Chain Associated Protein, p Tyrosine-protein kinase ZAP-70
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
A16043E_PECy7_ZAP70_Phospho-Tyr493_Antibody_v2_071421.png
Human peripheral blood lymphocytes were treated with (left), or without (right), hydrogen peroxide for five minutes, fixed with fixation buffer (Cat No. 420801), permeabilized with intracellular staining permeabilization wash buffer (Cat No. 421002), then intracellularly stained with anti-ZAP70 Phospho (Tyr493) (clone A16043E) PE/Cyanine7 and CD20 FITC.
  • A16043E_PECy7_ZAP70_Phospho-Tyr493_Antibody_v2_071421.png
    Human peripheral blood lymphocytes were treated with (left), or without (right), hydrogen peroxide for five minutes, fixed with fixation buffer (Cat No. 420801), permeabilized with intracellular staining permeabilization wash buffer (Cat No. 421002), then intracellularly stained with anti-ZAP70 Phospho (Tyr493) (clone A16043E) PE/Cyanine7 and CD20 FITC.
Compare all formats See PE/Cyanine7 spectral data
Cat # Size Price Save
396007 25 tests ¥37,620
396008 100 tests ¥90,420
Description

ZAP70 is a member of the Syk protein tyrosine kinase subfamily that is expressed exclusively in T cells and NK cells that plays an essential role in T cell receptor (TCR) signaling in combination with the Src family kinases, LCK and FYN. Upon TCR stimulation, phosphorylation of CD3 by LCK leads to the recruitment of ZAP70 to the activated receptor. Upon association of ZAP70 with the activated TCR complex, the kinase is phosphorylated on several tyrosine residues, including Tyrosine 493. Phosphorylation of Tyrosine 493, which is found within the activation loop of the protein, results in increased enzymatic activity. ZAP70 subsequently phosphorylates downstream targets, including the adaptor molecules LAT and LCP2, facilitating the recruitment and activation of other signaling molecules, ultimately leading to T cell proliferation, differentiation, and activation. ZAP70 mays also serve as a scaffold for recruiting additional factors to the activated TCR, and can function to regulate TCR-CD247/CD3Z expression at the plasma membrane. ZAP70 deficiency results in severely impaired immune function, while higher levels of ZAP70 expression are associated with certain B cell malignancies. PTN22 is required for the dephosphorylation of ZAP70 Tyrosine 493.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Positive staining is stimulation and donor dependent. Upon stimulation at high concentrations of H202 some staining of B cells can be seen. Staining for ZAP70 Phospho (Tyr493) using clone A16043E can also be successfully performed using the True-Nuclear™ Transcription Buffer Set and True-Phos™ Perm Buffer.

RRID
AB_2894506 (BioLegend Cat. No. 396007)
AB_2894506 (BioLegend Cat. No. 396008)

Antigen Details

Cell Type
NK cells, T cells
Biology Area
Cell Biology, Immunology, Signal Transduction
Molecular Family
Phospho-Proteins, Protein Kinases/Phosphatase, TCRs
Gene ID
7535 View all products for this Gene ID
UniProt
View information about ZAP70 Phospho Tyr493 on UniProt.org
Go To Top Version: 1    Revision Date: 07/14/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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