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PE/Cyanine7 anti-mouse Galectin-9 Antibody

Pricing & Availability
Clone
RG9-35 (See other available formats)
Regulatory Status
RUO
Other Names
Lgals9, Gal-9, Ecalectin
Isotype
Rat IgG2a, κ
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Product Citations
publications
RG9-35_PECy7_Galectin-9_Antibody_032019.png
C57BL/6 thymocytes were fixed and permeablized, then stained with anti-mouse Galectin-9 (clone RG9-35) PE/Cyanine7 (filled histogram) or Rat IgG2a, κ PE/Cyanine7 Isotype control (open histogram)
  • RG9-35_PECy7_Galectin-9_Antibody_032019.png
    C57BL/6 thymocytes were fixed and permeablized, then stained with anti-mouse Galectin-9 (clone RG9-35) PE/Cyanine7 (filled histogram) or Rat IgG2a, κ PE/Cyanine7 Isotype control (open histogram)
Compare all formats See PE/Cyanine7 spectral data
Cat # Size Price Save
136113 25 µg ¥36,300
136114 100 µg ¥82,500
Description

Galectin-9 is a mammalian lectin with a molecular weight of 40 kD that has two conserved carbohydrate recognition domains (CRDs) and forms homodimers. It recognizes N-acetyllactosamine (Galbeta1-4GlcNAc) and T-antigen (Galbeta1-3GalNAc). Tim-3 has been reported as its ligand. Galectin-9 is expressed by lymphocytes, dendritic cells, granulocytes, eosinophils, astrocytes, endothelial cells, fibroblasts, and thymus epithelial cells. It may be retained intracellularly or transported to the cell surface whereby cleavage generates a soluble form. Galectin-9 is involved in events such as cell aggregation, adhesion, chemotaxis, and apoptosis, and is important for the regulation of the immune response. Galectin-9 induces regulatory T cells, and suppresses Th1 and Th17 responses.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant mouse galectin-9
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is = 1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Fukushima A, et al. 2008. Int. Arch. Allergy Immunol. 146:36. (FA)
  2. Hou H, et al. 2014. PLoS One. 9:110585. PubMed
RRID
AB_2800640 (BioLegend Cat. No. 136113)
AB_2800640 (BioLegend Cat. No. 136114)

Antigen Details

Structure
Homodimer, each chain is a 343 aa protein of 40 kD that has two conserved carbohydrate recognition domains (CRDs)
Distribution
Lymphocytes, dendritic cells, neutrophils, eosinophils, astrocytes, endothelial cells, fibroblasts, thymus stromal/epithelial cells
Function
Cell aggregation, cell adhesion, chemotaxis, apoptosis, suppression of Th1 and Th17 responses, induction of regulatory T cells
Ligand/Receptor
Tim-3
Bioactivity
Chemotaxis, apoptosis, cell adhesion
Cell Type
Lymphocytes, Dendritic cells, Neutrophils, Eosinophils, Astrocytes, Endothelial cells, Fibroblasts, Epithelial cells, Tregs
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Adhesion, Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology
Molecular Family
Adhesion Molecules, Immune Checkpoint Receptors
Antigen References

1. Klibi J, et al. 2009. Blood 113:1957
2. Seki M, et al. 2008. Clin Immunol 127:78
3. Tsuboi Y, et al. 2007. Clin Immunol 124:221
4. Zhu C, et al. 2005. Nat Immunol 6:1245
5. Dunphy JL, et al. 2002. J. Biol. Chem. 277:14916

Gene ID
16859 View all products for this Gene ID
UniProt
View information about Galectin-9 on UniProt.org
Go To Top Version: 1    Revision Date: 03/20/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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