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PE/Cyanine7 anti-mouse IL-17A Antibody

Pricing & Availability
Clone
TC11-18H10.1 (See other available formats)
Regulatory Status
RUO
Other Names
Interleukin-17, Cytotoxic T lymphocyte-associated antigen 8 (CTLA-8)
Isotype
Rat IgG1, κ
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Product Citations
publications
TC11-18H10_PECy7_071009.jpg
PMA (20 ng/ml) + ionomycin (1 µg/ml) -stimulated (6 hours + monensin, 2 µM) mouse thymoma cell line EL-4 intracellularly stained with TC11-18H10.1 PE/Cyanine7
  • TC11-18H10_PECy7_071009.jpg
    PMA (20 ng/ml) + ionomycin (1 µg/ml) -stimulated (6 hours + monensin, 2 µM) mouse thymoma cell line EL-4 intracellularly stained with TC11-18H10.1 PE/Cyanine7
Compare all formats See PE/Cyanine7 spectral data
Cat # Size Price Save
506921 25 µg ¥26,170
506922 100 µg ¥61,770
Description

IL-17, also known as CTLA-8, is a T cell-expressed pleiotropic cytokine that exhibits a high degree of homology to a protein encoded by the ORF13 gene of herpes virus Saimiri. IL-17 is produced by Th cells (Th17) that are distinct from the traditional Th1- and Th2-cell subsets. IL-23 plays an important role in triggering IL-17 production. Both recombinant and natural IL-17 have been shown to exist as disulfide linked homodimers. IL-17 exhibits multiple biological activities on a variety of cells including: the induction of IL-6 and IL-8 production in fibroblasts, activation of NF-κB, and costimulation of T cell proliferation. IL-17 is an essential inflammatory mediator in the development of autoimmune diseases. Neutralization of IL-17 with monoclonal antibody is able to ameliorate the disease course.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
E. coli expressed, recombinant mouse IL-17A
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

ELISA Capture3,4 and ELISPOT Capture5: The purified TC11-18H10.1 antibody is useful as the capture antibody in a sandwich ELISA, when used in conjunction with the biotinylated TC11-8H4 antibody (Cat. No. 507002) as the detecting antibody and recombinant mouse IL-17 (Cat. No. 576009) as the standard.
Flow Cytometry2-4,7,8,11,12: The TC11-18H10.1 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-17-producing cells within mixed cell populations.
Neutralization6,9: The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of mouse IL-17 bioactivity in vivo and in vitro (Cat. No. 506906).

Application References

(PubMed link indicates BioLegend citation)
  1. Kennedy J, et al. 1996. J. Interferon Cytokine Res. 16:611.
  2. Schubert D, et al. 2004. J. Immunol. 172:4503. (ICFC)
  3. Infante-Duarte C, et al. 2000. J. Immunol. 165:6107. (ICFC, ELISA Capture)
  4. Harrington LE, et al. 2005. Nature Immunol. doi:10.1038/ni1254. (ICFC, ELISA Capture)
  5. Nekrasova T, et al. 2005. J. Immunol. 175:2734. (ELISPOT Capture)
  6. Yen D, et al. 2006. J. Clin. Invest. 116:1310. (Neut)
  7. Ehirchiou D, et al. 2007. J. Exp. Med. 204:1519. (ICFC)
  8. Kang SG, et al. 2007. J. Immunol. 179:3724. (ICFC)
  9. Smith E, et al. 2008. J. Immunol. 181:1357. (Neut) PubMed
  10. Neufert C, et al. 2007. Eur. J. Immunol. 37:1809. PubMed
  11. Wang C, et al. 2009. Mucosal Immunol 2:173. (ICFC) PubMed
  12. Cui Y, et al. 2009. Invest. Ophth. Vis. Sci. 50:5811. (ICFC) PubMed
  13. Kivisäkk P, et al. 2009. Ann. Neurol. 65:457. PubMed
  14. Cooney LA, et al. 2011. J. Immunol. 187:4440. PubMed
  15. Ma Y, et al. 2012. PLoS One. 7:e40763. PubMed
  16. Murakami R, et al. 2013. PLoS One. 8:73270. PubMed
Product Citations
  1. Han X, et al. 2022. Nutrients. 14:. PubMed
  2. Houlder E, et al. 2023. Nat Commun. 14:1863. PubMed
  3. Wang Y, et al. 2023. iScience. 26:106630. PubMed
  4. Vereertbrugghen A, et al. 2023. J Neuroinflammation. 20:120. PubMed
  5. Shao TY, et al. 2022. Cell Rep. 39:110837. PubMed
  6. Kumpunya S, et al. 2023. Front Immunol. 13:1010764. PubMed
  7. Bradley CP et al. 2017. Cell host & microbe. 22(5):697-704 . PubMed
  8. Komuczki J, et al. 2019. Immunity. 50:1289. PubMed
  9. Yu Y, et al. 2022. iScience. 25:105004. PubMed
  10. Mikami Y, et al. 2021. Immunity. 54(3):514-525.e6. PubMed
  11. Hewitson JP, et al. 2020. J Immunol. 204:2949. PubMed
  12. Turner JA, et al. 2020. Immunity. 53:1202. PubMed
  13. Kim CJ, et al. 2018. Immunity. 49:1034. PubMed
  14. Castellanos CA, et al. 2021. Sci Immunol. 6:eabh0707. PubMed
  15. Yang W, et al. 2020. Nat Commun. 3.553472222. PubMed
  16. Ferretti M, et al. 2016. Brain Behav Immun. 54: 211-225. PubMed
  17. Shrivastava T, et al. 2021. Front Immunol. 12:641447. PubMed
  18. Huang X, et al. 2020. Cell Host Microbe. 29(2):210-221.e6. PubMed
  19. Britton GJ et al. 2019. Immunity. 50(1):212-224 . PubMed
  20. Kang S, et al. 2021. Bio Protoc. 11:e4029. PubMed
  21. Hartwig T et al. 2018. Cell reports. 25(13):3564-3572 . PubMed
  22. Hum NR, et al. 2022. Front Oncol. 12:928474. PubMed
  23. Harb H, et al. 2021. Immunity. 54(6):1186-1199.e7. PubMed
  24. Bambouskova M, et al. 2021. Cell Reports. 34(10):108756. PubMed
  25. Jiang J, et al. 2017. Arthritis Res Ther. 10.1186/s13075-017-1261-9. PubMed
  26. Park S, et al. 2015. PLoS One. 10:129910. PubMed
  27. Place D, et al. 2017. PLoS One. 10.1371/journal.pone.0190384. PubMed
  28. Lima-Junior DS, et al. 2021. Cell. . PubMed
  29. Wagner A, et al. 2021. Cell. . PubMed
  30. Cai Y, et al. 2019. Cell Rep. 27:3034. PubMed
  31. Hao Y, et al. 2011. Blood. 118:1294. PubMed
  32. Denny JE, et al. 2019. Sci Rep. 2.786111111. PubMed
  33. Kim MY, et al. 2017. Plant Biotechnol J. 1.729166667. PubMed
  34. Guo S, et al. 2021. Sci Rep. 11:23745. PubMed
  35. Kolev M, et al. 2020. Immunity. 52(3):513-527. PubMed
  36. Miyauchi E, et al. 2020. Nature. 585:102. PubMed
  37. Roy S, et al. 2021. Nat Commun. 12:3182. PubMed
  38. Vogel A, et al. 2022. Cell Rep. 38:110420. PubMed
  39. Harb H, et al. 2020. Nat Immunol. 1359:21. PubMed
  40. Doss PMIA, et al. 2021. Cell Reports. 34(10):108833. PubMed
  41. Wu Y, et al. 2015. Biol Blood Marrow Transplant. 21: 1195-1204. PubMed
  42. Sparber F, et al. 2019. Cell Host Microbe. 25:389. PubMed
  43. Xu C, et al. 2021. Cell Reports. 35(11):109235. PubMed
  44. Mathur AN, et al. 2019. Immunity. 50:655. PubMed
  45. Wang X, et al. 2021. Immunity. 54(6):1123-1136.e8. PubMed
  46. Li X, et al. 2021. Front Immunol. 12:779560. PubMed
  47. Zheng QY, et al. 2020. FASEB J. 34:10590. PubMed
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RRID
AB_2125010 (BioLegend Cat. No. 506921)
AB_2125010 (BioLegend Cat. No. 506922)

Antigen Details

Structure
Cytokine; dimer; 15 kD (Mammalian).
Bioactivity
Secretion of IL-6, IL-8, G-CSF, prostaglandin E2 by epithelial, endothelial or fibroblastic cells; stimulates cell migration, cord formation, and IL-6 secretion by stromal cells
Cell Sources
CD4+ memory T cells
Cell Targets
Fibroblasts, epithelial and endothelial cells, stromal cells
Receptors
IL-17R (CD217)
Biology Area
Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Numasaki M, et al. 2002. Blood 101:2620.
3. Fossiez F, et al. 1996. J. Exp. Med. 183:2593.
4. Yao Z, et al. 1997. Cytokine 9:794.
5. Dong C. 2006. Nat. Rev. Immunol. 6:329.
6. Hofstetter HH, et al. 2005 Cell. Immunol. 237:123.

Gene ID
16171 View all products for this Gene ID
UniProt
View information about IL-17A on UniProt.org

Related FAQs

Does the anti-mouse IL-17 antibody (clone TC11-18H10.1) recognize the A or F isoform?
Clone TC11-18H10.1 recognizes IL-17A isoform, but it also recognizes the IL-17A/F heterodimer via IL-17A binding.

Other Formats

View All IL-17A Reagents Request Custom Conjugation
Description Clone Applications
PE anti-mouse IL-17A TC11-18H10.1 ICFC
Purified anti-mouse IL-17A TC11-18H10.1 ELISA Capture,CyTOF®,ELISPOT Capture,ICFC
FITC anti-mouse IL-17A TC11-18H10.1 ICFC
Alexa Fluor® 488 anti-mouse IL-17A TC11-18H10.1 ICFC
Alexa Fluor® 647 anti-mouse IL-17A TC11-18H10.1 ICFC
Alexa Fluor® 700 anti-mouse IL-17A TC11-18H10.1 ICFC
APC anti-mouse IL-17A TC11-18H10.1 ICFC
Pacific Blue™ anti-mouse IL-17A TC11-18H10.1 ICFC
PerCP/Cyanine5.5 anti-mouse IL-17A TC11-18H10.1 ICFC
PE/Cyanine7 anti-mouse IL-17A TC11-18H10.1 ICFC
Brilliant Violet 421™ anti-mouse IL-17A TC11-18H10.1 ICFC
Brilliant Violet 605™ anti-mouse IL-17A TC11-18H10.1 ICFC
Brilliant Violet 650™ anti-mouse IL-17A TC11-18H10.1 ICFC
Brilliant Violet 785™ anti-mouse IL-17A TC11-18H10.1 ICFC
Brilliant Violet 510™ anti-mouse IL-17A TC11-18H10.1 ICFC
Purified anti-mouse IL-17A (Maxpar® Ready) TC11-18H10.1 ELISA Capture,CyTOF®
PE/Dazzle™ 594 anti-mouse IL-17A TC11-18H10.1 ICFC
APC/Cyanine7 anti-mouse IL-17A TC11-18H10.1 ICFC
Brilliant Violet 711™ anti-mouse IL-17A TC11-18H10.1 ICFC
PerCP anti-mouse IL-17A TC11-18H10.1 ICFC
Ultra-LEAF™ Purified anti-mouse IL-17A TC11-18H10.1 ELISA Capture,CyTOF®,ELISPOT Capture,Neut,ICFC
PE/Cyanine5 anti-mouse IL-17A TC11-18H10.1 ICFC
Go To Top Version: 2    Revision Date: 10/16/2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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