PE/Cyanine7 anti-mouse TCR DO11.10 Antibody anti-TCR DO11.10

Clone: KJ1-26 (See other available formats)
Regulatory Status: RUO
Other Names: DO11.10 Clonotypic T Cell Receptor
Isotype: Mouse IgG2a, κ
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Product Citations: publications

KJ1-26_PECy7_TCRDO11.10 _Antibody_FC_080113.jpg — DO11.10 T-hybridoma cells were stained with TCR DO11.10 (clone KJ1-26) PE/Cyanine7 (filled histogram) or mouse IgG2a, κ PE/Cyanine7 isotype control (open histogram).

Compare all formats See PE/Cyanine7 spectral data

Cat #	Size	Price	Save
118515	25 µg	¥22,080
118516	100 µg	¥59,630

Description

DO11.10 transgenic TCR is specific for the chicken ovalbumin peptide (a.a. 323-339) presented on the I-A^d MHC class II complex. KJ1-26 antibody clone recognizes the DO11.10 TCR and enables identification of the DO11.10 TCR positive T-cells in the DO11.10 TCR transgenic mouse model. KJ1-26 antibody clone was reported to block the antigen response of DO11.10 T cell hybridomas in vitro.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: DO11.10 T hybridoma cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
RRID: AB_2562392 (BioLegend Cat. No. 118515)
AB_2562392 (BioLegend Cat. No. 118516)

Antigen Details

Structure: 80-90 kD TCR expressed on DO11.10 T hybridomas
Distribution: Clonotypic TCR
Ligand/Receptor: Ovalbumin peptide (aa 323-339) in the context of mouse MHC class II I-A^d
Biology Area: Immunology, Adaptive Immunity
Molecular Family: TCRs
Antigen References: 1. Haskins K, et al. 1983. J. Exp. Med. 157:1149.
2. Marrack P, et al. 1983. J. Exp. Med. 158:1635.
Gene ID: 21473 View all products for this Gene ID
UniProt: View information about TCR DO11.10 on UniProt.org

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Other Formats

View All TCR DO11.10 Reagents Request Custom Conjugation

Description	Clone	Applications
FITC anti-mouse TCR DO11.10	KJ1-26	FC
PE anti-mouse TCR DO11.10	KJ1-26	FC
PerCP/Cyanine5.5 anti-mouse TCR DO11.10	KJ1-26	FC
PE/Cyanine7 anti-mouse TCR DO11.10	KJ1-26	FC

Certificate of Analysis

Go To Top Version: 1 Revision Date: 08/09/2013

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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