PE/Dazzle™ 594 anti-human CD14 Recombinant Antibody, CD14, QA18A22

Clone: QA18A22 (See other available formats)
Regulatory Status: RUO
Other Names: LPS receptor
Isotype: Mouse IgG1, κ
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Product Citations: publications

QA18A22_PEDazzle594_CD14_Antibody_123020 — Human peripheral blood was treated with True-Stain Monocyte Blocker™ and stained with CD15 FITC and CD14 recombinant (clone QA18A22) PE/Dazzle™ 594 (left) or mouse IgG1, κ PE/Dazzle™ 594 isotype control (right).

Compare all formats See PE/Dazzle™ 594 spectral data

Cat #	Size	Price	Save
398713	25 tests	¥31,020
398714	100 tests	¥77,440

Description

CD14 is a 53-55 kD glycosylphosphatidylinositol (GPI)-linked membrane glycoprotein also known as LPS receptor. CD14 is expressed at high levels on monocytes and macrophages, and at lower levels on granulocytes. Some dendritic cell populations such as interfollicular dendritic cells, reticular dendritic cells, and Langerhans cells have also been reported to express CD14. As a high-affinity receptor for LPS, CD14 is involved in the clearance of gram-negative pathogens and in the upregulation of adhesion molecules and cytokines expression in monocytes and neutrophils.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Human
Antibody Type: Recombinant
Host Species: Mouse
Immunogen: Proprietary
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE/Dazzle™ 594 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* PE/Dazzle™ 594 has a maximum excitation of 566 nm and a maximum emission of 610 nm.
Excitation Laser: Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
RRID: AB_2890846 (BioLegend Cat. No. 398713)
AB_2890846 (BioLegend Cat. No. 398714)

Antigen Details

Structure: GPI-linked membrane glycoprotein, 53-55 kD
Distribution: Monocytes, macrophages, granulocytes (low)
Function: LPS receptor, clearance of Gram-negative pathogens
Ligand/Receptor: LPS
Cell Type: Granulocytes, Macrophages, Monocytes, Neutrophils
Biology Area: Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family: CD Molecules
Antigen References: Stocks S, et al. 1990. Biochem. J. 268:275.

Wright S, et al. 1990. Science 249:1434.
Gene ID: 929 View all products for this Gene ID
UniProt: View information about CD14 on UniProt.org

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Description	Clone	Applications
PE anti-human CD14 Recombinant Antibody	QA18A22	FC
Purified anti-human CD14 Recombinant Antibody	QA18A22	FC
APC/Cyanine7 anti-human CD14 Recombinant Antibody	QA18A22	FC
APC anti-human CD14 Recombinant Antibody	QA18A22	FC
PerCP/Cyanine5.5 anti-human CD14 Recombinant Antibody	QA18A22	FC
PE/Cyanine7 anti-human CD14 Recombinant Antibody	QA18A22	FC
PE/Dazzle™ 594 anti-human CD14 Recombinant Antibody	QA18A22	FC

Certificate of Analysis

Go To Top Version: 1 Revision Date: 12/30/2020

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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