PE/Dazzle™ 594 anti-human CD162 Antibody, CD162, KPL-1

Clone: KPL-1 (See other available formats)
Regulatory Status: RUO
Other Names: PSGL-1, p-selectin glycoprotein ligand-1
Isotype: Mouse IgG1, κ
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Product Citations: publications

Human peripheral blood lymphocytes were stained with CD3 APC and CD162 (clone KPL-1) PE/Dazzle™ 594 (left) or Mouse IgG1, κ PE/Dazzle™ 594 isotype control (right).

Compare all formats See PE/Dazzle™ 594 spectral data

Cat #	Size	Price	Save
328819	25 tests	¥37,620
328820	100 tests	¥69,740

Description

CD162, also known as p-selectin glycoprotein ligand-1 (PSGL-1), is a 120 - 220 kD, mucin-like type I transmembrane glycoprotein. CD162 binds to CD62P (P-Selectin), CD62E (E-Selectin) and CD62L (L-Selectin). The interactions between P-selectin and P-selectin glycoprotein ligand-1 (PSGL-1) mediate the earliest "rolling" of leukocytes on the lumenal surface of activated endothelium, and the interaction between leukocytes and activated platelets or other leukocytes found at sites of inflammation. CD162 is expressed on neutrophils, monocytes, and most lymphocytes including NK and T cells but PSGL-1 stains B cells at significantly lower levels than other cell types.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Human, Cynomolgus, Rhesus
Reported Reactivity: African Green, Baboon
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: PSGL-1 transfected murine 300.19 pre B-cell line
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE/Dazzle™ 594 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Clone KPL-1 is reported to recognize the tyrosine sulfation consensus motif of PSGL-1¹. Additional reported applications (for the relevant formats) include: Western Blot¹, immunoprecipitation², immunohistochemical staining of acetone-fixed frozen tissue sections and formalin-fixed paraffin embedded tissue sections¹, blocks the recognition of PSGL-1 with P- and L-selectin¹.
RRID: AB_2810500 (BioLegend Cat. No. 328819)
AB_2810500 (BioLegend Cat. No. 328820)

Antigen Details

Distribution: Neutrophils, monocytes, and most lymphocytes
Function: Mediates the earliest "rolling" of leukocytes on the lumenal surface of activated endothelium, and the interaction between leukocytes and activated platelets or other leukocytes found at sites of inflammation.
Ligand/Receptor: CD62P (P-Selectin), CD62E (E-Selectin) and CD62L (L-Selectin)
Cell Type: Lymphocytes, Monocytes, Neutrophils
Biology Area: Cell Adhesion, Immunology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Snapp KR, et al. 1998. Blood 91:154.
Gene ID: 6404 View all products for this Gene ID
UniProt: View information about CD162 on UniProt.org

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Cell Surface Flow Cytometry Staining Protocol

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Other Formats

View All CD162 Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-human CD162	KPL-1	FC,IHC-P,IHC-F,IP,WB
PE anti-human CD162	KPL-1	FC
Brilliant Violet 421™ anti-human CD162	KPL-1	FC,IHC-P
APC anti-human CD162	KPL-1	FC
APC/Fire™ 750 anti-human CD162	KPL-1	FC
Alexa Fluor® 647 anti-human CD162	KPL-1	FC,IHC-P
PE/Cyanine7 anti-human CD162	KPL-1	FC
PerCP/Cyanine5.5 anti-human CD162	KPL-1	FC
PE/Dazzle™ 594 anti-human CD162	KPL-1	FC
TotalSeq™-A0871 anti-human CD162	KPL-1	PG
TotalSeq™-B0871 anti-human CD162 Antibody	KPL-1	PG
TotalSeq™-C0871 anti-human CD162	KPL-1	PG

Certificate of Analysis

Go To Top Version: 1 Revision Date: 05/01/2019

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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