PE/Fire™ 810 anti-mouse CD3 Antibody, CD3, 17A2

Clone: 17A2 (See other available formats)
Regulatory Status: RUO
Other Names: T cell antigen receptor complex, T3
Isotype: Rat IgG2b, κ
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Product Citations: publications

17A2_PEFire810_CD3_Antibody_112921 — C57BL/6 splenocytes were stained with anti-mouse/human CD45R/B220 FITC and anti-mouse CD3 (clone 17A2) PE/Fire™ 810 (left) or CD45R/B220 FITC only (right).

Compare all formats See PE/Fire™ 810 spectral data

Cat #	Size	Price	Save
100277	25 µg	¥34,980

Description

CD3, also known as T3, is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3 is composed of CD3ε, δ, γ and ζ chains. It forms a TCR complex by associating with TCR α/β or γ/δ chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex

Product Details

Technical data sheet

Product Details

Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: γδTCR-positive T-T hybridoma D1
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography and conjugated with PE/Fire™ 810 under optimal conditions.
Concentration: 0.2 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality Tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PE/Fire™ 810 has a maximum excitation of 488/561 nm and a maximum emission of 810 nm.

Excessive exposure to light, and commonly used fixation, permeabilization buffers can affect PE/Fire™ 810 fluorescence signal intensity and spread. Please keep conjugates protected from light exposure. For more information and representative data, visit our Fire Dyes page.
Excitation Laser: Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Additional reported application (for relevant formats) include: spatial biology (IBEX)^1,2.
RRID: AB_2910273 (BioLegend Cat. No. 100277)

Antigen Details

Structure: Ig superfamily, CD3/TCR, 20 kD
Distribution: Thymocytes (differentiation dependent), mature T cells, NK-T cells
Function: Antigen recognition, TCR signal transduction, T cell activation
Ligand/Receptor: Peptide antigen/MHC-complex
Antigen References: 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Davis MM. 1990. Annu. Rev. Biochem. 59:475.
3. Weiss A, et al. 1994. Cell 76:263.
Gene ID: 12502 View all products for this Gene ID
UniProt: View information about CD3 on UniProt.org

Documentation

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Other Formats

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Description	Clone	Applications
FITC anti-mouse CD3	17A2	FC
PE anti-mouse CD3	17A2	FC
Purified anti-mouse CD3	17A2	FC,IHC-F,IP,ICC
Alexa Fluor® 647 anti-mouse CD3	17A2	FC,IHC-F,3D IHC,SB
Alexa Fluor® 488 anti-mouse CD3	17A2	FC,IHC-F,3D IHC
Pacific Blue™ anti-mouse CD3	17A2	FC
Alexa Fluor® 700 anti-mouse CD3	17A2	FC
PerCP/Cyanine5.5 anti-mouse CD3	17A2	FC
PE/Cyanine7 anti-mouse CD3	17A2	FC
APC/Cyanine7 anti-mouse CD3	17A2	FC
Brilliant Violet 421™ anti-mouse CD3	17A2	FC,ICC
Brilliant Violet 570™ anti-mouse CD3	17A2	FC
Brilliant Violet 650™ anti-mouse CD3	17A2	FC
Brilliant Violet 785™ anti-mouse CD3	17A2	FC
Brilliant Violet 510™ anti-mouse CD3	17A2	FC
APC anti-mouse CD3	17A2	FC
Ultra-LEAF™ Purified anti-mouse CD3	17A2	FC,IHC-F,IP,ICC
Brilliant Violet 605™ anti-mouse CD3	17A2	FC
Alexa Fluor® 594 anti-mouse CD3	17A2	IHC-F,FC,3D IHC,SB
Brilliant Violet 711™ anti-mouse CD3	17A2	FC
Biotin anti-mouse CD3	17A2	FC,IHC-F
PE/Dazzle™ 594 anti-mouse CD3	17A2	FC
APC/Fire™ 750 anti-mouse CD3	17A2	FC
Brilliant Violet 750™ anti-mouse CD3	17A2	FC
TotalSeq™-A0182 anti-mouse CD3	17A2	PG
TotalSeq™-B0182 anti-mouse CD3	17A2	PG
Spark Blue™ 550 anti-mouse CD3	17A2	FC
Spark NIR™ 685 anti-mouse CD3	17A2	FC
TotalSeq™-C0182 anti-mouse CD3	17A2	PG
APC/Fire™ 810 anti-mouse CD3	17A2	FC
PE/Fire™ 640 anti-mouse CD3	17A2	FC
Spark YG™ 570 anti-mouse CD3	17A2	IHC-F
PE/Fire™ 700 anti-mouse CD3	17A2	FC
PE/Cyanine5 anti-mouse CD3	17A2	FC
Spark Blue™ 574 anti-mouse CD3 Antibody	17A2	FC
Spark Violet™ 423 anti-mouse CD3	17A2	FC
PE/Fire™ 810 anti-mouse CD3	17A2	FC
Spark Red™ 718 anti-mouse CD3	17A2	FC
Spark UV™ 387 anti-mouse CD3	17A2	FC

Certificate of Analysis

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Go To Top Version: 2 Revision Date: 05/03/2023

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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