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PE Recombinant Protein L

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Regulatory Status
RUO
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Protein-L_-PE_FC_1_060123
Human peripheral blood mononuclear cell (PBMC) lymphocytes were stained with anti-human CD19 APC and PE Recombinant Protein L (left) or stained with anti-human CD19 APC only (right).
  • Protein-L_-PE_FC_1_060123
    Human peripheral blood mononuclear cell (PBMC) lymphocytes were stained with anti-human CD19 APC and PE Recombinant Protein L (left) or stained with anti-human CD19 APC only (right).
See PE spectral data
Cat # Size Price Save
303651 50 tests ¥49,500
Description

Protein L was initially isolated from bacteria Peptostreptococcus magnus cell wall, and now is prepared as recombinant protein. Protein L has been found to have a high affinity for Ig κ light chains. This unique ability of protein L to bind to the κ light chains of antibodies makes it valuable in detection and purification of antibodies and antibody fragments that contain kappa light chains but not the Fc region, such as antigen-binding VL domains and Fv fragments prepared from monoclonal antibodies. It is also used to detect chimeric antigen receptor (CAR) expressing cells.

Product Details
Technical data sheet

Product Details

Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

For multi-color flow cytometry analysis, cells should be stained with Protein L first, and washed; costaining reagent(s) need to be applied as the second step.  

Antigen Details

Cell Type
B cells
Biology Area
Cell Biology, Immuno-Oncology, Immunology
Antigen References
  1. Björck L. 1988. J Immunol. 140:1194-7.
  2. Akerström B and Björck L. 1989. J Biol Chem. 264:19740-6.
Gene ID
NA
UniProt
View information about Protein L on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 05/09/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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