PE/Fire™ 640 anti-human CD45RO Antibody, CD45RO, UCHL1

Clone: UCHL1 (See other available formats)
Regulatory Status: RUO
Workshop: IV N31
Other Names: CD45RO
Isotype: Mouse IgG2a, κ
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Product Citations: publications

Human peripheral blood lymphocytes were stained with CD45RA FITC and CD45RO (clone UCHL1) PE/Fire™ 640 (left), or CD45RA FITC only (right).

Compare all formats See PE/Fire™ 640 spectral data

Cat #	Size	Price	Save
304263	25 tests	¥38,720
304264	100 tests	¥84,040

Description

CD45RO is a 180 kD single chain membrane glycoprotein. It is a splice variant of tyrosine phosphatase CD45, lacking the A, B, and C determinants. The CD45RO isoform is expressed on activated and memory T cells, some B cell subsets, activated monocytes/macrophages, and granulocytes. CD45RO enhances both T cell receptor and B cell receptor signaling mediated activation. CD45 and its isoforms non-covalently associate with lymphocyte phosphatase-associated phosphoprotein (LPAP) on T and B lymphocytes. CD45 has been reported to be associated with several other cell surface antigens including CD1, CD2, CD3, and CD4. CD45 has also been reported to bind galectin-1 and CD22. CD45 isoform expression can change in response to cytokines.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Human
Reported Reactivity: Chimpanzee, Cynomolgus, Common Marmoset
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: IL-2 dependent T cell line, CA1
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE/Fire™ 640 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* PE/Fire™ 640 has a maximum excitation of 566 nm and a maximum emission of 639 nm.
Excitation Laser: Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: The UCHL1 antibody is commonly used in combination with antibodies against CD45RA to discern memory and naïve T cells. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections⁵ and formalin-fixed paraffin-embedded tissue sections⁴, Western blotting², and immunoprecipitation³.
RRID: AB_2876599 (BioLegend Cat. No. 304263)
AB_2876599 (BioLegend Cat. No. 304264)

Antigen Details

Structure: Tyrosine phosphatases, type I transmembrane, 180 kD (isoform of CD45 containing none of the A, B, or C determinants)
Distribution: Activated and memory T cells, B cell subsets, monocytes, macrophages, granulocytes
Function: Enhances TCR and BCR signaling
Ligand/Receptor: CD22
Cell Type: B cells, Granulocytes, Macrophages, Mesenchymal Stem Cells, Monocytes, T cells, Tregs
Biology Area: Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family: CD Molecules
Antigen References: 1. Thomas M. 1989. Annu. Rev. Immunol. 7:339.
2. Trowbridge I, et al. 1994. Annu. Rev. Immunol. 12:85.
Gene ID: 5788 View all products for this Gene ID
UniProt: View information about CD45RO on UniProt.org

Documentation

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Cell Surface Flow Cytometry Staining Protocol

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Other Formats

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Description	Clone	Applications
APC anti-human CD45RO	UCHL1	FC
FITC anti-human CD45RO	UCHL1	FC
PE anti-human CD45RO	UCHL1	FC,SB
PE/Cyanine5 anti-human CD45RO	UCHL1	FC
Purified anti-human CD45RO	UCHL1	FC,WB,IP,IHC-P,SB
Alexa Fluor® 488 anti-human CD45RO	UCHL1	FC,IHC-P
Pacific Blue™ anti-human CD45RO	UCHL1	FC
Alexa Fluor® 700 anti-human CD45RO	UCHL1	FC
Biotin anti-human CD45RO	UCHL1	FC
Brilliant Violet 421™ anti-human CD45RO	UCHL1	FC,IHC-P
PerCP/Cyanine5.5 anti-human CD45RO	UCHL1	FC
Brilliant Violet 570™ anti-human CD45RO	UCHL1	FC
Brilliant Violet 605™ anti-human CD45RO	UCHL1	FC
APC/Cyanine7 anti-human CD45RO	UCHL1	FC
PE/Cyanine7 anti-human CD45RO	UCHL1	FC
Brilliant Violet 650™ anti-human CD45RO	UCHL1	FC
Brilliant Violet 711™ anti-human CD45RO	UCHL1	FC
Brilliant Violet 785™ anti-human CD45RO	UCHL1	FC
Alexa Fluor® 594 anti-human CD45RO	UCHL1	IHC-P
Purified anti-human CD45RO (Maxpar® Ready)	UCHL1	FC,CyTOF®
Brilliant Violet 510™ anti-human CD45RO	UCHL1	FC
PE/Dazzle™ 594 anti-human CD45RO	UCHL1	FC
APC/Fire™ 750 anti-human CD45RO	UCHL1	FC
PerCP anti-human CD45RO	UCHL1	FC
APC anti-human CD45RO	UCHL1	FC
TotalSeq™-A0087 anti-human CD45RO	UCHL1	PG
TotalSeq™-B0087 anti-human CD45RO	UCHL1	PG
TotalSeq™-C0087 anti-human CD45RO	UCHL1	PG
Brilliant Violet 750™ anti-human CD45RO	UCHL1	FC
PE/Fire™ 640 anti-human CD45RO	UCHL1	FC
TotalSeq™-D0087 anti-human CD45RO	UCHL1	PG
Spark Violet™ 423 anti-human CD45RO	UCHL1	FC
GMP APC anti-human CD45RO	UCHL1	FC
PE anti-human CD45RO	UCHL1	FC
TotalSeq™-Bn1369 anti-human CD45RO	UCHL1	SB
GMP PE anti-human CD45RO	UCHL1	FC
Spark UV™ 387 anti-human CD45RO	UCHL1	FC
APC/Fire™ 810 anti-human CD45RO	UCHL1	FC

Certificate of Analysis

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Go To Top Version: 2 Revision Date: 08/28/2020

For Research Use Only. Not for diagnostic or therapeutic use.

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