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PE/Fire™ 700 anti-human CD62L Antibody

Pricing & Availability
Clone
W21031N (See other available formats)
Regulatory Status
RUO
Other Names
L-selectin, LECAM-1, LAM-1, Leu-8, TQ-1
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W21031N_PEFire700_CD62L_Antibody_1_031124
Human peripheral blood lymphocytes were stained with anti-human CD3 (clone UCHT1) FITC and anti-human CD62L (clone W21031N) PE/Fire™ 700 (left) or rat IgG2a, κ PE/Fire™ 700 isotype control (right).
  • W21031N_PEFire700_CD62L_Antibody_1_031124
    Human peripheral blood lymphocytes were stained with anti-human CD3 (clone UCHT1) FITC and anti-human CD62L (clone W21031N) PE/Fire™ 700 (left) or rat IgG2a, κ PE/Fire™ 700 isotype control (right).
Compare all formats See PE/Fire™ 700 spectral data
Cat # Size Price Save
384817 25 tests ¥37,400
384818 100 tests ¥82,500
Description

CD62L is a 74-95 kD single chain type I glycoprotein referred to as L-selectin or LECAM-1. It is expressed on most peripheral blood B cells, subsets of T and NK cells, monocytes, granulocytes, and certain hematopoietic malignant cells. CD62L binds to carbohydrates present on certain glycoforms of CD34, glycam-1, and MAdCAM-1 and with a low affinity to anionic oligosaccharide sequences related to sialylated Lewis X (sLex, CD15s) through its C-type lectin domain. CD62L is important for the homing of naïve lymphocytes to high endothelial venules in peripheral lymph nodes and Peyer's patches. It also plays a role in leukocyte rolling on activated endothelial cells.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant human CD62L protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE/Fire™ 700 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* PE/Fire™ 700 has a maximum excitation of 565 nm and a maximum emission of 695 nm.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Clone W21031N can completely block the binding of clone DREG-56 on target cells. Clone W21031N does not affect the binding of clone S21001C on target cells.

RRID
AB_3106075 (BioLegend Cat. No. 384817)
AB_3106075 (BioLegend Cat. No. 384818)

Antigen Details

Structure
Selectin, single chain glycoprotein, 74-95 kD
Distribution

Majority of B cells, naïve T cells, subset of memory T and NK cells, monocytes, granulocytes, thymocytes

Function
Leukocyte homing, leukocyte tethering, rolling
Ligand/Receptor
CD34, GlyCAM, MAdCAM-1
Cell Type
B cells, Granulocytes, Lymphocytes, Monocytes, Neutrophils, NK cells, T cells, Thymocytes, Tregs
Biology Area
Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References
  1. Kishimoto T, et al. 1990. Proc Natl Acad Sci U S A. 87:2244-8.
  2. Kishimoto T, et al. 1991. Blood. 78:805-11.
Gene ID
6402 View all products for this Gene ID
UniProt
View information about CD62L on UniProt.org
Go To Top Version: 1    Revision Date: 03/11/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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