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PerCP/Cyanine5.5 anti-Asialo-GM1 Antibody

Pricing & Availability
Clone
Poly21460 (See other available formats)
Regulatory Status
RUO
Other Names
Ganglioside, GM1, ganglio-N-tetraosylceramide
Isotype
Rabbit Polyclonal IgG
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Product Citations
publications
Poly21460_PerCPCyanine55 _Asialo-GM1_Antibody_040924
C57BL/6 mouse splenocytes were stained with NK1.1 FITC and anti-Asialo-GM1 (clone Poly21460) PerCP/Cyanine5.5.
  • Poly21460_PerCPCyanine55 _Asialo-GM1_Antibody_040924
    C57BL/6 mouse splenocytes were stained with NK1.1 FITC and anti-Asialo-GM1 (clone Poly21460) PerCP/Cyanine5.5.
Compare all formats See PerCP/Cyanine5.5 spectral data
Cat # Size Price Save
146009 25 µg ¥40,260
146010 100 µg ¥95,480
Description

GM1 is a ganglioside, a type of glycosphingolipid with a single sialic acid group. Asialo-GM1 is a GM1 derivative without a sialic acid group. It is expressed on NK cells, basophils, monocytes/macrophages, and T cells. It is particularly expressed on very early thymocytes, but the expression decreases as the cells mature and become Thy-1+. The highest expression is detected on neuronal tissues. This molecule has been shown to be involved in microbial pathogenesis. Antibodies specific for Asialo-GM1 are elevated in dementia, lupus, and Guillain-Barré syndrome.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse, Rat
Antibody Type
Polyclonal
Host Species
Rabbit
Immunogen
Asialo-GM1
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is = 0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Excitation Laser
Blue Laser (488 nm)
Application Notes

This antibody recognizes asialo-GM1. It does not react with other glycolipids, such as GM1 and Asialo-GM2.

This antibody can partially block IL-12 induced IFN-? production but does not affect other systemic action of IL-12. 


This product may contain other non-IgG subtypes.

Application References

(PubMed link indicates BioLegend citation)
  1. Naiki M, et al. 1974. J. Immunol. 113:84.
  2. Kasai M, et al. 1981. Nature. 291:334. (Depletion)
  3. Nishikado H, et al. 2011. J. Immunol. 186:5766. (Depletion)
  4. Zhou G, et al. 2013. Eur. J. Immunol. 43:929. (Depletion)
RRID
AB_2728172 (BioLegend Cat. No. 146009)
AB_2728172 (BioLegend Cat. No. 146010)

Antigen Details

Structure
Asialo-GM1 is a GM1 derivative without a sialic acid group, with the structure: βDGalp(1-3)βDGalpNAc(1-4)βDGalp(1-4)βDGlcp(1-1)Cer
Distribution

NK cells, basophils, splenic T lymphocytes, highest concentrations on neurons

Function
Supports cellular identification, neuronal plasticity, and repair mechanisms
Interaction
Facilitates cellular recognition and cell-to-cell communication
Ligand/Receptor
Ligand for cholera toxin
Cell Type
Basophils, Neurons, NK cells, T cells
Biology Area
Cell Biology, Immunology, Innate Immunity, Signal Transduction
Antigen References

1. Stein-Douglas K, et al. 1979. J. Exp. Med. 143:822. 
2. Kasai M, et al. 1980. Eur. J. Immunol. 10:175. 
3. Young WW Jr, et al. 1980. J. Immunol. 124:199. 
4. Bansal AS, et al. 1994. J. Clin. Path. 47:300.

Gene ID
NA
UniProt
View information about Asialo-GM1 on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 1    Revision Date: 03/13/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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