Loading...

PerCP/Cyanine5.5 anti-human CD371 (CLEC12A) Antibody

Pricing & Availability
Clone
50C1 (See other available formats)
Regulatory Status
RUO
Workshop
HCDM listed
Other Names
CLL1, MICL, CLL-1, DCAL-2, C-type lectin domain family 12 member A, CD371
Isotype
Mouse IgG2a, κ
Ave. Rating
Submit a Review
Product Citations
publications
50C1_PerCPCyanine55_CD371_Antibody_110218
Human peripheral blood monocytes were stained with CD371 (CLEC12A, clone 50C1) PerCP/Cyanine5.5 (filled histogram), or mouse IgG2a, κ PerCP/Cyanine5.5 isotype control (open histogram).
  • 50C1_PerCPCyanine55_CD371_Antibody_110218
    Human peripheral blood monocytes were stained with CD371 (CLEC12A, clone 50C1) PerCP/Cyanine5.5 (filled histogram), or mouse IgG2a, κ PerCP/Cyanine5.5 isotype control (open histogram).
Compare all formats See PerCP/Cyanine5.5 spectral data
Cat # Size Price Save
353611 25 tests ¥38,720
353612 100 tests ¥84,040
Description

CD371 (CLEC12A), also known as DCAL-2, MICL or CLL-1, is a 30 kD type II transmembrane protein with extracellular C-type lectin domains, belonging to the C-type lectin family.  It is expressed on monocytes, granulocytes, NK cells, and basophils.  Its cytoplasmic ITIM motif modulates signaling cascades and is involved in phosphorylation of tyrosine residues in MAP kinases.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
293T cells expressing CLEC12A-Flag
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Excitation Laser
Blue Laser (488 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Lahoud MH, et al. 2009. J. Immunol. 182:7587. (FC)
  2. Lahoud MH, et al. 2011. J. Immunol. 187:842. (FC)
  3. Neumann K, et al. 2014. Immunity. 40:389. PubMed
RRID
AB_2565544 (BioLegend Cat. No. 353611)
AB_2565544 (BioLegend Cat. No. 353612)

Antigen Details

Structure
Type II C-type lectin family member, 265 aa, 30.7 kD
Distribution

Monocytes, granulocytes, NK cells, and basophils

Function
Negative regulator of granulocyte and monocyte function
Interaction
PTPN6 and PTPN11
Cell Type
Basophils, Dendritic cells, Granulocytes, Monocytes, NK cells
Biology Area
Immunology, Costimulatory Molecules
Molecular Family
CD Molecules
Antigen References

1. Lahoud MH, et al. 2009. J. Immunol. 182:7587.
2. Chen CH, et al. 2006. Blood 107:1459.
3. Marshall AS, et al. 2004. J. Biol. Chem. 279:14792.

Gene ID
160364 View all products for this Gene ID
UniProt
View information about CD371 on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 2    Revision Date: 11/02/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account