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PerCP/Cyanine5.5 anti-human ROR1 Antibody

Pricing & Availability
Clone
2A2 (See other available formats)
Regulatory Status
RUO
Other Names
Receptor tyrosine kinase-like orphan receptor 1, Tyrosine-protein kinase transmembrane receptor ROR1, Neurotrophic tyrosine kinase receptor-related 1, NTRKR1
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
2A2_PerCPCyanine55_ROR1_Antibody_110718
Human teratocarcinoma cell line NCCIT was stained with ROR1 (clone 2A2) PerCP/Cyanine5.5 (filled histogram) or mouse IgG1, κ PerCP/Cyanine5.5 isotype control (open histogram).
  • 2A2_PerCPCyanine55_ROR1_Antibody_110718
    Human teratocarcinoma cell line NCCIT was stained with ROR1 (clone 2A2) PerCP/Cyanine5.5 (filled histogram) or mouse IgG1, κ PerCP/Cyanine5.5 isotype control (open histogram).
Compare all formats See PerCP/Cyanine5.5 spectral data
Cat # Size Price Save
357809 25 tests ¥38,720
357810 100 tests ¥84,040
Description

ROR1, also known as NTRKR1, is a type I transmembrane protein and member of the ROR subfamily of surface receptors. ROR1 consists of one frizzled domain, one Ig-like C2-type domain, one kringle domain, and one kinase domain with no catalytic activity. ROR1 is expressed on embryonic tissue, in the central nervous system and on some cancer cells, and is used as a marker for B-cell chronic lymphocytic leukemia. Wnt5a has been identified as a ligand for ROR1.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Clone 2A2 binds to the membrane distal Ig-domain of ROR1. Clone 2A2 has also been shown to exhibit cross reactivity towards mouse ROR12.

Application References

(PubMed link indicates BioLegend citation)
  1. Dave H, et al. 2012. PLoS One 7:e52655. (FC)
  2. Baskar S, et al. 2012. MAbs. 4:349. (FC)
RRID
AB_2563277 (BioLegend Cat. No. 357809)
AB_2563277 (BioLegend Cat. No. 357810)

Antigen Details

Structure
Type I transmembrane protein, consist of 1 frizzled domain, 1 Ig-like C2-type domain, 1 kringle domain, and 1 non-functional kinase domain; member of the ROR subfamily of surface receptors
Distribution

Embryonic tissue, central nervous system, chronic lymphocytic leukemia cells

Ligand/Receptor
Wnt5a
Cell Type
Embryonic Stem Cells, Leukemia
Biology Area
Immunology, Stem Cells
Antigen References
  1. Bicocca VT, et al. 2012. Cancer Cell. 22:656.
  2. Zhang S, et al. 2012. PLoS One 7:e31127.
  3. Uhrmacher S, et al. 2011. Leuk Res. 35:1360.
  4. Yang J, et al. 2011. PLoS One 6:e21018.
  5. Grumolato L, et al. 2010. Genes Dev. 24:2517.
Gene ID
4919 View all products for this Gene ID
UniProt
View information about ROR1 on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 3    Revision Date: 11/07/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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