- Clone
- 551 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Sialic acid-binding immunoglobulin-like lectin
- Isotype
- Rat IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![551_PerCPCy5.5_1_090310 551_PerCPCy5.5_1_090310](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/551_PerCPCy5.5_1_090310.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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C57BL/6 splenocytes stained with CD45R/B220 (RA3-6B2) Alexa Fluor® 647and 551 PerCP/Cyanine5.5 -
C57BL/6 splenocytes stained with CD45R/B220 (RA3-6B2) Alexa Fluor® 647 and rat IgG1 PerCP/Cyanine5.5 isotype control
Cat # | Size | Price | Save |
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129613 | 25 µg | ¥27,280 | |
129614 | 100 µg | ¥73,700 |
The Siglec-H is a member of CD33-related Siglec family which mostly expressed in the innate immune system. Siglec-H is reported to be specifically expressed on plasmacytoid dendritic cells. Siglec-H depends on adaptor protein DAP 12 (DNAX-activating protein of 12 kD) for surface expression and signal transduction. Cross-linking with this antibody can selectively inhibit interferon-alpha production by IPCs following TLR9 ligation.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Siglec-H-Fc recombinant fusion protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography, and conjugated withPerCP/Cyanine5.5 under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Additional Product Notes
- BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
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Application References
(PubMed link indicates BioLegend citation) -
- Blasius AL, et al. 2006. J. Immunol. 177:3260.
- Product Citations
-
- RRID
-
AB_10639936 (BioLegend Cat. No. 129613)
AB_10643995 (BioLegend Cat. No. 129614)
Antigen Details
- Structure
- Sialic acid-binding immunoglobulin-like lectins
- Distribution
-
Siglec-H is expressed exclusively on natural interferon-producing cells (IPCs), also called plasmacytoid dendritic cells in spleen, blood and lymph nodes. The IPCs are characterized by a complex phenotype (CD11c+, Ly-6C+, B220+, CD11b-, CD8+/-).
- Function
- Siglec-H associates with adaptor protein DAP12 for cell signaling, which reduces type 1 IFN response of IPC to CpG.
- Ligand/Receptor
- Sialic acid
- Cell Type
- Dendritic cells
- Biology Area
- Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- CD Molecules, Siglec Molecules
- Antigen References
-
1. Blasius AL, et al. 2006. Blood 107:2474.
2. Zhang J, et al. 2006. Blood 107:3600.
3. Blasius AL, et al. 2007. J. Exp. Med. 204:2561. - Gene ID
- 233274 View all products for this Gene ID
- UniProt
- View information about Siglec H on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
- How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
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PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.
Other Formats
View All Siglec H Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-mouse Siglec H | 551 | FC |
FITC anti-mouse Siglec H | 551 | FC |
PE anti-mouse Siglec H | 551 | FC |
Alexa Fluor® 647 anti-mouse Siglec H | 551 | FC |
APC anti-mouse Siglec H | 551 | FC |
Pacific Blue™ anti-mouse Siglec H | 551 | FC |
PerCP/Cyanine5.5 anti-mouse Siglec H | 551 | FC |
TotalSeq™-A0119 anti-mouse Siglec H | 551 | PG |
TotalSeq™-C0119 anti-mouse Siglec H | 551 | PG |
TotalSeq™-B0119 anti-mouse Siglec H | 551 | PG |
Spark PLUS UV™ 395 anti-mouse Siglec H | 551 | FC |
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-mouse Siglec H
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FITC anti-mouse Siglec H
C57BL/6 splenocytes stained with B220 (RA3-6B2) PE and 551 F... -
PE anti-mouse Siglec H
C57BL/6 splenocytes stained with B220 (RA3-6B2) FITC and 551... -
Alexa Fluor® 647 anti-mouse Siglec H
C57BL/6 splenocytes stained with RA3-6B2 (B220) PE and 551 A... -
APC anti-mouse Siglec H
C57BL/6 splenocytes stained with B220 (RA3-6B2) PE and 551 A... C57BL/6 splenocytes stained with B220 (RA3-6B2) PE and rat I... -
Pacific Blue™ anti-mouse Siglec H
C57BL/6 splenocytes stained with B220 (RA3-6B2) PE and 551 P... C57BL/6 splenocytes stained with B220 (RA3-6B2) PE and rat I... -
PerCP/Cyanine5.5 anti-mouse Siglec H
C57BL/6 splenocytes stained with CD45R/B220 (RA3-6B2) Alexa ... C57BL/6 splenocytes stained with CD45R/B220 (RA3-6B2) Alexa ... -
TotalSeq™-A0119 anti-mouse Siglec H
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TotalSeq™-C0119 anti-mouse Siglec H
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TotalSeq™-B0119 anti-mouse Siglec H
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Spark PLUS UV™ 395 anti-mouse Siglec H
C57BL/6 mouse splenocytes were stained with anti-mouse/human...
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