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PerCP/Cyanine5.5 anti-rat CD11b/c Antibody

Pricing & Availability
Clone
OX-42 (See other available formats)
Regulatory Status
RUO
Other Names
Rat C3bi receptor, Rat CR3 complement receptor, ITGAM, ITGAX
Isotype
Mouse IgG2a, κ
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Product Citations
publications
OX-42_PerCPCyanine55_CD11bslashc_Antibody_111918
LOU rat splenocytes were stained with CD11b/c (clone OX-42) PerCP/Cyanine5.5 (filled histogram) or mouse IgG2a, κ PerCP/Cyanine5.5 isotype control (open histogram).
  • OX-42_PerCPCyanine55_CD11bslashc_Antibody_111918
    LOU rat splenocytes were stained with CD11b/c (clone OX-42) PerCP/Cyanine5.5 (filled histogram) or mouse IgG2a, κ PerCP/Cyanine5.5 isotype control (open histogram).
Compare all formats See PerCP/Cyanine5.5 spectral data
Cat # Size Price Save
201819 25 µg ¥28,380
201820 100 µg ¥62,040
Description

The OX-42 antibody reacts with the CR3 complement (C3bi) receptor expressed on monocytes, granulocytes, macrophages, dendritic cells, and NK cells. This antibody appears to recognize a common epitope shared between CD11b and CD11c (integrin αM and αX chains). The OX-42 antibody precipitates three polypeptides with apparent molecular weights of 160, 103, and 95 kD, respectively. This antibody has been shown to block the formation of complement-mediated rosettes and leukocyte migration.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Rat peritoneal macrophages.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is =0.125 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen sections1,2, immunoprecipitation3, in vivo and in vitro blocking of C3bi binding3,4.

Application References

(PubMed link indicates BioLegend citation)
  1. Whiteland JL, et al. 1995. J. Histochem. Cytochem. 43:313. (IHC)
  2. Milligan CE, et al. 1991. J. Comp. Neurol. 314:125. (IHC)
  3. Robinson AP, et al. 1986. Immunology 57:239. (Block)
  4. Issekutz SE, et al. 1992. Immunology 76:655. (Block)
  5. Muehlbauer SM, et al. Am. J Pathol. 177:735. (FC) PubMed
  6. Thomas RA. et al. 2009. J Pharmacol Toxicol Methods. 60:263. PubMed
  7. Takeda Y, et al. 2011. Immunobiology. 216:1094. PubMed
  8. Furuhashi K, et al. 2013. J Am Soc Nephrol. PubMed
  9. Cheng HY, et al. 2013. Cytotherapy. 1465:608. PubMed
Product Citations
  1. Zhang G, et al. 2023. Front Pharmacol. 14:1159075. PubMed
  2. Shen W, et al. 2023. Dis Model Mech. 16: . PubMed
  3. Li L, et al. 2023. Sci Adv. 9:eade1067. PubMed
  4. Liu C, et al. 2022. J Inflamm Res. 15:2803. PubMed
  5. Neumann B, et al. 2019. Cell Stem Cell. 25:473. PubMed
  6. de la Fuente AG, et al. 2020. Mol Cell Proteomics. 19:1281. PubMed
  7. de la Fuente AG, et al. 2020. Mol Cell Proteomics. 1.68125. PubMed
  8. DU T, et al. 2020. Front Immunol. 1.571527778. PubMed
RRID
AB_2565949 (BioLegend Cat. No. 201819)
AB_2565949 (BioLegend Cat. No. 201820)

Antigen Details

Structure
Integrin family member
Distribution

Monocytes, granulocytes, macrophages, dendritic cells

Function
Receptor for C3bi
Ligand/Receptor
Complement C3bi
Cell Type
Dendritic cells, Granulocytes, Macrophages, Monocytes
Biology Area
Cell Biology, Complement, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Robinson AP, et al. 1986. Immunology 57:239.
2. Barcaly AN. 1981 Immunology 42:593.

Gene ID
25021 View all products for this Gene ID 499271 View all products for this Gene ID
UniProt
View information about CD11b CD11c on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 2    Revision Date: 11/19/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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