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PerCP/Fire™ 780 anti-mouse CD62L Antibody

Pricing & Availability
Clone
W18021D (See other available formats)
Regulatory Status
RUO
Other Names
L-selectin
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W18021D_PerCPFire780_CD62L_Antibody_1_100924-2
C57BL/6 mouse splenocytes were stained with anti-mouse CD3ε (clone 145-2C11) APC and anti-mouse CD62L (clone W18021D) PerCP/Fire™ 780 (left) or rat IgG2a, κ PerCP/Fire™ 780 isotype control (right).
  • W18021D_PerCPFire780_CD62L_Antibody_1_100924-2
    C57BL/6 mouse splenocytes were stained with anti-mouse CD3ε (clone 145-2C11) APC and anti-mouse CD62L (clone W18021D) PerCP/Fire™ 780 (left) or rat IgG2a, κ PerCP/Fire™ 780 isotype control (right).
Compare all formats See PerCP/Fire™ 780 spectral data
Cat # Size Price Save
161223 25 µg ¥42,680
161224 100 µg ¥95,480
Description

CD62L is a 74-95 kD glycoprotein also known as L-selectin. It is a member of the selectin family and is expressed on the majority of B and naïve T cells, a subset of memory T cells, monocytes, granulocytes, most thymocytes, and a subset of NK cells. CD62L is important in lymphocyte homing to high endothelial venules (HEV) in peripheral lymph nodes and leukocyte "rolling" on activated endothelium. CD62L also contributes to neutrophil emigration at inflammatory sites. CD62L is rapidly shed from lymphocytes and neutrophils upon cellular activation and the expression levels of CD62L (in conjunction with other markers) have been used to distinguish naïve, effector, and memory T cells. CD62L has been reported to interact with CD34, GlyCAM-1, and MAdCAM-1.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse CD62L recombinant protein (39-332)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP/Fire™ 780 under optimal conditions.
Concentration
0.2 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Fire™ 780 has a maximum excitation of 478 nm and a maximum emission of 780 nm.

Excitation Laser
Blue Laser (488 nm)

Antigen Details

Structure
CD62L is a type I transmembrane glycoprotein with 74-95 kD.
Distribution

CD62L is expressed on the majority of B and naïve T cells, a subset of memory T cells, monocytes, granulocytes, most thymocytes, and a subset of NK cells.

Function
CD62L plays roles in lymphocyte homing to lymphoid organs and inflammatory leukocyte trafficking.
Ligand/Receptor
CD34, GlyCAM-1, MAdCAM-1
Cell Type
B cells, Granulocytes, Monocytes, Neutrophils, NK cells, T cells, Tregs
Biology Area
Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Rosen S. 2004. Annu. Rev. Immunol. 22:129
2. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.

Gene ID
20343 View all products for this Gene ID
UniProt
View information about CD62L on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 10/09/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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