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Purified anti-A2M Antibody

Pricing & Availability
Clone
A21084F (See other available formats)
Regulatory Status
RUO
Other Names
alpha-2-macroglobulin (α2M or A2M), C3 and PZP-like alpha-2-macroglobulin domain-containing protein (CPAMD5), transcuprein
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
A. A21084F_PURE_A2M_WB_102524
Whole cell extracts (15 µg total protein per lane) from the indicated cell lines/tissues were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with Purified anti-A2M (clone A21084F) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG (Cat. No. 405405). Direct-Blot™ HRP anti-β-actin (Cat. No. 664804) was used as a loading control at a 1:10000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • A. A21084F_PURE_A2M_WB_102524
    Whole cell extracts (15 µg total protein per lane) from the indicated cell lines/tissues were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with Purified anti-A2M (clone A21084F) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG (Cat. No. 405405). Direct-Blot™ HRP anti-β-actin (Cat. No. 664804) was used as a loading control at a 1:10000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • B. A21084F_PURE_A2M_IHC-P_102524
    IHC staining with Purified anti-A2M (clone A21084F) on formalin-fixed paraffin-embedded human liver tissue. Following antigen retrieval using Tris-EDTA (Cat. No. 422703), the tissue was incubated without (panel A) or with (panel B) Purified anti-A2M (clone A21084F) followed by incubation with Alexa Fluor° 647 Goat anti-rat IgG (Cat. No. 405416) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801). Images were captured with a 40X objective. Scale bar: 50 µm
  • C. A21084F_PURE_A2M_ICFC_102524
    Hep G2 cells (positive control, filled histogram) and SH-SY5Y cells (low expression control, open histogram) were fixed and permeabilized using True-Phos™ Perm Buffer (Cat. No. 425401) and intracellularly stained with either Purified anti-A2M (clone A21084F), or Purified rat IgG2b, κ isotype control (open histogram, dashed line) (representative histogram for both cell lines) (Cat. No. 400602), followed by Alexa Fluor 647® Goat anti-mouse IgG (Cat. No. 405322).
Cat # Size Price Save
649851 25 µg ¥41,800
649852 100 µg ¥107,800
Description

Alpha-2-macroglobulin (A2M) is a large plasma protein that plays a crucial role in the immune system and homeostasis. Secreted primarily by the liver, A2M mainly functions as a broad-spectrum protease inhibitor, capable of trapping and neutralizing a wide variety of proteases. This action is protective against excessive tissue damage during inflammation and other immune responses. A2M also has roles in binding and clearing various cytokines, growth factors, and other bioactive molecules from the bloodstream, thereby regulating their activity. Finally, A2M is also involved in the clearance of protein fragments and other cellular debris, contributing to overall tissue repair and maintenance. Due to its role in protein clearance and inflammation modulation, A2M may also play a role in the progression of Alzheimer’s disease.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant full-length human A2M
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P, ICFC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1.0 - 10.0 µg/mL is suggested. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody (clone A21084F) does not cross-react with mouse A2M in Western Blot.

For use in immunohistochemistry on formalin-fixed paraffin-embedded tissue (IHC-P), it is recommended to perform antigen retrieval using either Citrate Buffer, 10X (Cat. No. 420902) or Tris-EDTA pH 9.0 Antigen Retrieval Buffer (10X) (Cat. No. 422704).

This product is not recommended for use in immunocytochemistry (ICC).

For use in intracellular flow cytometry (ICFC), fixation with fixation buffer (Cat. No. 420801) and permeabilization with True-PhosTM Perm Buffer (Cat. No. 425401) is recommended.

Antigen Details

Structure
A2M is a 1474 amino acid protein with a predicted molecular weight of 163.3 kD
Distribution

Secreted

Function
Protease inhibitor
Interaction
Circulating A2M primarily exists as a homotetramer of A2M subunits, although dimeric and monomeric forms have been described. A2M binds to a wide variety of proteases.
Molecular Family
Enzymes and Regulators, Proteases
Antigen References
  1. Vandooren J, Itoh Y 2021. Front. Immunol. 12:803244.
  2. Rehman AA, et al. 2013. J Cell Physiol. 228(8):1665-75.
  3. Garcia-Ferrer I, et al.2017. Subcell Biochem. 83:149-183.
Gene ID
2 View all products for this Gene ID
UniProt
View information about A2M on UniProt.org

Other Formats

View All A2M Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-A2M A21084F WB,ICFC,IHC-P
Go To Top Version: 1    Revision Date: 10/25/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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