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- Clone
- W16012A (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Aryl hydrocarbon receptor, Ah receptor, AHR, Ah, Ahh, Ahre, bHLHe76
- Isotype
- Rat IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
Whole cell extracts (15 µg protein) from NIH3T3 cells transfected with non-targeting control siRNA (siCON) or siRNA targeting Ahr (siAhr) were resolved on a 4-12% Bis-Tris gel, transferred to PVDF and probed with 1.0 µg/mL (1:500 dilution) of Purified anti-AHR Antibody, clone W16012A, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular Weight marker.
The Aryl hydrocarbon receptor (AHR) is a member of the basic helix–loop–helix–PER– ARNT–SIM (bHLH–PAS) subgroup of the bHLH superfamily of transcription factors and is the only member of this family known to be activated by ligands. Those ligands can be endogenous, naturally occurring, and/or anthropogenic. In vitro studies and gene expression profiles showed that the transcriptional changes by the activated Ahr are ligand specific and are highly cell specific. There is only one ligand binding pocket in the AHR (in the Per-ARNT-Sim-B domain), and the affinity of the best characterized high affinity ligand, TCDD (2,3,7,8-tetrachlorodibenzo-pdioxin), depends on only a few amino acids in that pocket. The Ahr can also be activated by numerous stress factors and substances that may not fit into the binding pocket, such as hyperoxia, oxidized low-density lipoproteins, hydrogen peroxide, ozone, and metals. Ahr regulates genes involved in a wide variety of biochemical pathways, including energy metabolism, lipid and cholesterol synthesis, xenobiotic metabolism and various transporters. The Ahr is well known to be involved in a variety of cellular processes, such as the cell cycle, epithelial barrier function, cell migration, and immune function.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Partial mouse Ahr (635-848 a.a.) expressed in E. coli.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
KO/KD-WB - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
Clone W16012A does not cross-react with human (in-house tested).
- Product Citations
-
- RRID
-
AB_2650731 (BioLegend Cat. No. 694502)
Antigen Details
- Structure
- 848 amino acids with a predicted molecular weight of 95 kD.
- Distribution
-
Cytoplasm and nucleus.
- Function
- Ligand-activated transcriptional activator. Binds to the XRE promoter region of genes it activates. Activates the expression of multiple phase I and II xenobiotic chemical metabolizing enzyme genes (such as the CYP1A1 gene). Mediates biochemical and toxic effects of halogenated aromatic hydrocarbons. Involved in cell-cycle regulation. Regulates the circadian clock by inhibiting the basal and circadian expression of the core circadian component PER1. Inhibits PER1 by repressing the CLOCK-ARNTL/BMAL1 heterodimer mediated transcriptional activation of PER1.
- Interaction
- In the nucleus, heterodimer of AHR and ARNT. Interacts with coactivators including SRC-1, RIP140 and NOCA7, and with the corepressor SMRT. Interacts with MYBBP1A, NEDD8, ARNTL/BMAL1.
- Ligand/Receptor
- Hydrophobic aromatic compound.
- Biology Area
- Cell Biology, Transcription Factors
- Antigen References
-
1. Bersten DC, et al. 2013. Nat. Rev. Cancer 13:827.
2. Murray IA, et al. 2014. Nat. Rev. Cancer 14:801.
3. Reisz-Porszasz S, et al. 1994. Mol. Cell. Biol. 14(9):6075.
4. Esser C, Rannug A. 2015. Pharmacol. Rev. 67:259.
5. Sun YV, et al. 2004. Nucleic Acids Res. 32:4512.
6. Whelan F, et al. 2010. Mol. Pharmacol. 77:1037. - Gene ID
- 11622 View all products for this Gene ID
- UniProt
- View information about Ahr on UniProt.org
Related FAQs
Other Formats
View All Ahr Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-Ahr | W16012A | WB,KO/KD-WB |
| PE anti-Ahr | W16012A | ICFC |
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