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- Clone
- A15127A (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Synuclein alpha-140, non-A4 component of amyloid, alpha-synuclein, isoform NACP140, non-amyloid beta component (NAP), PARK1, NACP, PD1
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
IHC staining of α-synuclein deposits with purified anti-α-Synuclein, C-Terminal Truncated antibody (clone A15127A) on formalin-fixed, paraffin-embedded Parkinson's disease brain tissue. Following antigen retrieval using 70% formic acid, the tissue was incubated with the primary antibody at 5 µg/mL overnight at 4°C. BioLegend's Ultra-Streptavidin (USA) HRP kit was used for detection followed by hematoxylin counterstaining, according to the protocol provided. -
Direct ELISA of purified anti-α-Synuclein, C-Terminal Truncated antibody (clone A15127A) binding to plate-immobilized recombinant human full-length (1-140) and C-terminally fragmented (1-122) α-synuclein. ELISA was performed by coating wells with 150 ng of each recombinant α-synuclein protein. The wells were then incubated with the primary antibody at 37°C for one hour, followed by incubation with horseradish peroxidase labeled goat anti-mouse secondary antibody. TMB (3, 3', 5, 5' tetramethylbenzidine, Cat. No. 421501) was used as the detection system.
| Cat # | Size | Price | Save |
|---|---|---|---|
| 848401 | 25 µg | ¥19,580 | |
| 848402 | 100 µg | ¥47,960 |
Alpha-synuclein is expressed principally in the nervous system, but it is also produced in other tissues, including the skin. In the brain, the protein is primarily neuronal, but it is also present in glia. Neuronal α-synuclein is concentrated in presynaptic nerve terminals, interacts with plasma membrane phospholipids, and is also present in nuclei and mitochondria. At least three isoforms of α-synuclein are produced through alternative splicing. The most common isoform is a 140 amino acid-long transcript. Other isoforms include, α-synuclein-126, lacking residues 41-54; and α-synuclein-112, which lacks residues 103-130. α-synuclein’s physiological role is poorly understood, but the protein has been implicated in regulating dopamine release and transport, synaptic vesicle clustering, and functioning as a SNARE-complex chaperone. α-synuclein fibrils are a major component of the intracellular Lewy bodies that are associated with Parkinson's disease, Lewy body dementia, and multiple system atrophy. Phosphorylation of α-synuclein at Tyr39 may modulate clearance of the protein and contribute to Parkinson's disease.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- KLH-conjugated synthetic peptide of α-synuclein with C-terminus terminating at Asn122.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
IHC-P - Quality tested
Direct ELISA - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 2.0 - 10 µg/ml is suggested. For Direct ELISA, a concentration range of 0.02 - 3.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
- RRID
-
AB_2650690 (BioLegend Cat. No. 848401)
AB_2650690 (BioLegend Cat. No. 848402)
Antigen Details
- Structure
- α-synuclein's canonical isoform is a 140 amino acid protein with an apparent molecular mass of 14 kD.
- Distribution
-
Tissue sources: Primarily nervous system, but lower expression in other tissues.
Distribution: Cytoskeleton, cytosol, lysosome, mitochondria, nucleus, plasma membrane, and extracellular. - Biology Area
- Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
- Molecular Family
- α-Synuclein
- Antigen References
-
1. Mor DE, et al. 2016. Neurobiol Dis. 88:66. PubMed
2. Jucker M & Walker LC. 2013. Nature. 501(7465):45. PubMed
3. Bartels T, et al. 2011. Nature. 477(7362): 107. PubMed
4. Devine MJ, et al. 2011. Mov Disord. 26:2160. PubMed
5. Mishizen-Eberz AJ et al. 2003. J Neurochem. 86:836. PubMed
6. Games D et al. 2014. J Neurosci. 34(28):9441. PubMed - Gene ID
- 6622 View all products for this Gene ID
- UniProt
- View information about alpha-Synuclein on UniProt.org
Related FAQs
Other Formats
View All α-Synuclein, (C-Terminal Truncated x-122) Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-α-Synuclein (C-Terminal Truncated x-122) | A15127A | IHC-P,Direct ELISA |
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Purified anti-α-Synuclein (C-Terminal Truncated x-122)
IHC staining of α-synuclein deposits with purified anti-α-Sy... 
Direct ELISA of purified anti-α-Synuclein, C-Terminal Trunca...
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