- Clone
- Syn514 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Synuclein alpha-140, non-A4 component of amyloid, alpha-synuclein, isoform NACP140, non-A beta component of AD amyloid Parkinson disease (autosomal dominant, Lewy body) 4
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Synucleins are small soluble proteins expressed primarily in neural tissues and in certain tumors. The family includes 3 known proteins: alpha-, beta-, and gamma-synuclein. All synucleins have in common a highly conserved alpha helical lipid binding motif with similarity to the class-A2 lipid binding domains of the exchangeable apolipoproteins. The alpha- and beta-synuclein proteins are found primarily in brain tissue, where they are seen mainly in the pre-synaptic terminals. Alpha-synuclein is believed to be a major component of Lewy bodies in Parkinson's disease. Mutations in alpha-synuclein are associated with rare familial cases of early onset Parkinson's disease (PD), Alzheimer's disease (AD) and several other neurodegenerative illnesses. Oxidative injury has been implicated in the pathogenesis of neurodegenerative disorders including AD and PD.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Syn514 was raised using oxidized alpha-synuclein (α-synuclein) and recognizes its native and nitrated/oxidized forms.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
IHC-P - Quality tested
ICC - Reported in the literature, not verified in house - Recommended Usage
-
Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 0.5 - 5.0 µg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
Additional reported applications (for the relevant formats) include: immunofluorescent staining3,7.
Syn514 also shows some cross reactivity with beta- and gamma-synuclein. -
Application References
(PubMed link indicates BioLegend citation) -
- Duda JE, et al. 2002. Ann. Neurol. 52(2):205-10.
- Giasson BI, et al. 2000. Science 290:985.
- Waxman EA, Giasson BI. 2008. J. Neuropathol. Exp. Neurol. 5:402. (IF)
- Daher JP, et al. 2014. Proc. Natl. Acad. Sci. USA 25:9289. (IHC)
- Uryu K, et al. 2003. Exp. Neurol. 1:214. (IHC)
- Auluck PK, et al. 2002. Science 5556:865. (IHC)
- Mazzulli JR, et al. 2007. J. Biol. Chem. 43:31621. (IF)
- RRID
-
AB_2616740 (BioLegend Cat. No. 838903)
Antigen Details
- Structure
- Alpha synuclein is 140 amino acids and has a molecular mass of 14 kD.
- Distribution
-
Cytoplasm, nucleus, and extracellular.
- Biology Area
- Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
- Molecular Family
- α-Synuclein
- Gene ID
- 6622 View all products for this Gene ID
- UniProt
- View information about alpha-Synuclein on UniProt.org
Related Pages & Pathways
Pages
Other Formats
View All α-Synuclein, Nitrated Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-α-Synuclein, Nitrated | Syn514 | IHC-P,ICC |
Compare Data Across All Formats
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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Purified anti-α-Synuclein, Nitrated
IHC staining of Syn514 on formalin-fixed paraffin-embedded h...
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