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Purified anti-AMD1 Antibody

Pricing & Availability
Clone
W18041A (See other available formats)
Regulatory Status
RUO
Other Names
Adenosylmethionine Decarboxylase 1, SAMDC, S-Adenosylmethionine Decarboxylase 1, S-Adenosylmethionine Decarboxylase Proenzyme, AdoMetDC
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
a. W18041A_PURE_AMD1_WB_102424
Whole cell extracts (15 µg total protein per lane) from indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with Purified anti-AMD1 (clone W18041A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG (Cat. No. 405405). Direct-Blot™ HRP anti-β-actin (Cat. No. 664804) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • a. W18041A_PURE_AMD1_WB_102424
    Whole cell extracts (15 µg total protein per lane) from indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with Purified anti-AMD1 (clone W18041A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG (Cat. No. 405405). Direct-Blot™ HRP anti-β-actin (Cat. No. 664804) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • B. W18041A_PURE_AMD1_IHC-P_102424
    IHC staining with Purified anti-AMD1 (clone W18041A) on formalin-fixed paraffin-embedded human testis tissue. Following antigen retrieval using 1X Tris-EDTA pH 9.0 Antigen Retrieval Buffer (Cat. No. 422704), the tissue was incubated with (panel A) and without (panel B) Purified anti-AMD1 (clone W18041A) followed by incubation with Alexa Fluor® 647 Goat anti-rat IgG (Cat. No. 405416) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801). Images were captured with a 40X objective and merged (panels C and D). Scale bar: 50 µm
Cat # Size Price Save
660851 25 µg ¥34,100
660852 100 µg ¥95,700
Description

AMD1 (Adenosylmethionine Decarboxylase 1) is an enzyme critical for polyamine biosynthesis, a process vital for cell growth, proliferation, and differentiation. It decarboxylates S-adenosylmethionine (SAM) to form decarboxylated SAM (dcSAM), a crucial step in the synthesis of spermidine and spermine, which plays a key role in stabilizing cellular structures like DNA and RNA.

AMD1 maintains normal cell function through polyamine regulation, while its dysregulation is associated with cancer, neurological diseases, and inflammation. In many cancers, such as colon, prostate, and breast cancer, AMD1 is overexpressed. This leads to elevated polyamine levels, promoting rapid cell division, uncontrolled growth, and tumor progression. Given its role in driving tumor growth, AMD1 is viewed as a potential target for cancer therapy. Inhibiting AMD1 may decrease polyamine production, thereby slowing cancer progression.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant fragment of human AMD1
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 5.0 - 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

For use in immunohistochemistry on formalin-fixed paraffin-embedded tissue (IHC-P), it is recommended to perform antigen retrieval using either Citrate Buffer, 10X (Cat. No. 420902) or Tris-EDTA pH 9.0 Antigen Retrieval Buffer (10X) (Cat. No. 422704).

It is not recommended using this product in immunocytochemistry (ICC) or intracellular flow cytometry (ICFC).

This antibody does not cross react with mouse AMD1 in Western Blot.

Antigen Details

Structure
Heterotetramer of two alpha and two beta chains; AMD1 is a 334 amino acid length protein with a predicted molecular weight of 38.3 kD.
Distribution

Widely expressed in tissues. Located in the cytoplasm

Function
Essential for biosynthesis of the polyamines spermidine and spermine
Biology Area
Cell Proliferation and Viability, DNA Repair/Replication
Molecular Family
Enzymes and Regulators
Gene ID
262 View all products for this Gene ID
UniProt
View information about AMD1 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 10/24/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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