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Purified anti-ATF6 Antibody

Pricing & Availability
Clone
W17028A (See other available formats)
Regulatory Status
RUO
Other Names
Cyclic AMP-dependent transcription factor ATF-6 alpha, ATF-6 alpha, Activating transcription factor 6 alpha, ATF6-alpha
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W17028A_Pure_ATF6_Antibody_1_041118
Western blot of anti-ATF6 antibody (clone W17028A). Lane 1: Molecular weight marker; Lane 2: 20 µg of Hela cell lysate; Lane 3: 20 µg of lysate from Hela cells treated with 4 mM DTT for 2 hours; Lane 4: 20 µg of SH-SY5Y cell lysate; Lane 5: 20 µg of lysate from SH-SY5Y cells treated with 4 mM DTT for 2 hours; Lane 6: 1 ng of His-tagged human ATF6 α; Lane 7: 1 ng of His-tagged human ATF6 β. The blot was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-Rat IgG (Cat. No. 405405). Enhanced chemiluminescence was used as the detection system.
  • W17028A_Pure_ATF6_Antibody_1_041118
    Western blot of anti-ATF6 antibody (clone W17028A). Lane 1: Molecular weight marker; Lane 2: 20 µg of Hela cell lysate; Lane 3: 20 µg of lysate from Hela cells treated with 4 mM DTT for 2 hours; Lane 4: 20 µg of SH-SY5Y cell lysate; Lane 5: 20 µg of lysate from SH-SY5Y cells treated with 4 mM DTT for 2 hours; Lane 6: 1 ng of His-tagged human ATF6 α; Lane 7: 1 ng of His-tagged human ATF6 β. The blot was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-Rat IgG (Cat. No. 405405). Enhanced chemiluminescence was used as the detection system.
  • W17028A_Pure_ATF6_Antibody_2_041118
    ICC staining of anti-ATF6 antibody (clone W17028A) on Hela cells. The cells were treated with (bottom) or without (top) 4 mM DTT for 2 hours, fixed with 4% PFA, permeabilized with a buffer containing 0.1% Triton X-100 and 0.25% BSA, and blocked with 2% normal goat serum and 0.02% BSA. The cells were then incubated with 5 µg/ml of the primary antibody overnight at 4°C, followed by incubation with 5 µg/ml of Alexa Fluor® 594 goat anti-Rat IgG (Cat. No. 405422) for one hour at room temperature. Nuclei were counterstained with DAPI. The images were captured with a 40X objective. Scale bar: 50 µm
Cat # Size Price Save
853101 25 µg ¥24,640
853102 100 µg ¥60,720
Description

ATF6 encodes a transcription factor that is anchored in the endoplasmic reticulum (ER) and activated during the unfolded protein response (UPR) to protect cells from ER stress. Under conditions of ER stress, ATF6 is transported from the ER to the Golgi apparatus, where it is cleaved by Golgi-resident proteases to release the cytosolic DNA-binding portion. Then the processed ATF6 moves to the nucleus to activate gene expression. Deletion of the isoform activating transcription factor 6α (ATF6α) and its paralog ATF6β results in embryonic lethality and notochord dysgenesis in nonhuman vertebrates, and loss-of-function mutations in ATF6α are associated with malformed neuroretina and congenital vision loss in humans. Altered ATF6 function in neurodegenerative disorders such as Parkinson's disease has been observed. 

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Human ATF6α recombinant protein (1-250 a.a.) expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 5.0 µg per ml. For immunocytochemistry, a concentration range of 5.0 - 10.0 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody specifically recognizes ATF6a but not ATF6ß, verified by WB.

Product Citations
  1. Caddeo C, et al. 2023. Int J Mol Sci. 24:. PubMed
  2. Domenger A, et al. 2022. EMBO Mol Med. 14:e14740. PubMed
  3. Chaudhary P, et al. 2021. J Cell Biochem. 122:1886. PubMed
  4. Rinaldi R, et al. 2021. Cells. 10:. PubMed
  5. Shi J, et al. 2022. J Virol. 96:e0169521. PubMed
  6. Lu IT, et al. 2022. Mar Drugs. 20:. PubMed
RRID
AB_2728605 (BioLegend Cat. No. 853101)
AB_2728605 (BioLegend Cat. No. 853102)

Antigen Details

Structure
ATF6α is a 670 amino acid protein with a molecular weight mass of ~80 kD.
Distribution

Tissue distribution: ATF6 is ubiquitously expressed in numerous cell types including cells in of the central nervous system (CNS).
Cellular distribution: Cytoplasmic, nucleus, ER, and golgi apparatus.

Function
ATF6 is a transmembrane glycoprotein that functions as a transcription activator and initiates the unfolded protein response during ER stress.
Interaction
ATF6 interacts with ER chaperone immunoglobulin-binding protein (BiP), and can be cleaved by lumenal site 1 protease (S1P) and intra-membrane site 2 protease (S2P).
Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Neuroscience Cell Markers, Protein Trafficking and Clearance, Transcription Factors
Molecular Family
Endoplasmic Reticulum Markers
Antigen References
  1. Shen J, et al. 2002. Dev Cell. 3:99-111
  2. Ron D and Walter P. 2007. Nat Rev Mol Cell Biol. 8: 519-529
  3. Credle JJ, et al. 2015. Neurobiol Dis. 76: 112-125
  4. Jin JK, et al. 2017. Circ Res. 120: 862-875
  5. Kroeger H, et al. 2018. Sci Signal. 11: 5785
Gene ID
22926 View all products for this Gene ID
UniProt
View information about ATF6 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 06/09/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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