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Purified anti-AU1 Epitope Tag Antibody (Previously Covance catalog# MMS-130P)

Pricing & Availability
Clone
AU1 (See other available formats)
Regulatory Status
RUO
Other Names
AU1 epitope tag, DTYRYI epitope tag, DTYRYI tag
Previously
Covance Catalog# MMS-130P
Isotype
Mouse IgG2a
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Product Citations
publications
Cat # Size Price Save
901905 25 µL ¥19,580
901901 200 µL ¥78,320
901902 500 µL ¥122,320
Description

The AU1 tag, which consists of the peptide sequence DTYRYI, is often used as a protein modification in order to simplify the labeling and detection of proteins. This unique amino acid sequence allows for specific antibody detection in western blotting and other immunostaining techniques. Due to the short sequence, this modification is not likely to affect the structure or function of the modified proteins.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Epitope tag
Reported Reactivity
Species independent
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Monoclonal antibody AU1 was raised against an SDS-disrupted BPV-1 preparation and mapped to the six amino acid epitope DTYRYI.
Formulation
Phosphate-buffered solution + 0.03% Thimerosal.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application

WB, ICC, IP, Purification
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting.

The optimal working dilution should be determined for each specific assay condition.
• WB: 1:1,000
• ICC: 1:1,000
• IP: 1:150

Application Notes

This antibody is effective in immunofluorescence (IF), immunoblotting (WB), and immunoprecipitation (IP) of tagged proteins.

The clone was specifically designed for use in epitope tagging. Because of the small size of the epitope, it is unlikely to alter the activity of the cloned sequence. This antibody was purified using protein-G chromatography.

Application References

(PubMed link indicates BioLegend citation)
  1. Mothe-Satney I, et al. 2000. J Biol Chem. 275:33836.
  2. Goldstein DJ, et al. 1992. EMBO J. 11:4851.
  3. Lim PS, et al. 1990. J Infect Dis. 162:1263.
  4. Sauter D, et al. 2011. PLoS Pathog. 8:e1003093. (WB, IF)
  5. Kluge SF, et al. 2013. Retrovirology 10:32. (WB, IF) PubMed
  6. Heigele A, et al. 2012. J. Virol. 86:36. WB) PubMed
Product Citations
  1. Heigele A, et al. 2012. J Virol. 86:36-48. PubMed
  2. Waise TMZ, et al. 2019. Nat Commun. 10:714. PubMed
  3. Wroblewska A et al. 2018. Cell. 175(4):1141-1155 . PubMed
  4. Kluge S, et al. 2013. Retrovirology. 10:32. PubMed
  5. Kim YJ, et al. 2021. PLoS Pathog. 17:e1009179. PubMed
  6. Chen X, et al. 2021. Sci Rep. 11:14525. PubMed
  7. Joas S, et al. 2020. Cell Rep. 30:2261. PubMed
RRID
AB_2565014 (BioLegend Cat. No. 901905)
AB_2565014 (BioLegend Cat. No. 901901)
AB_2565014 (BioLegend Cat. No. 901902)

Antigen Details

Biology Area
Cell Biology
Gene ID
NA
UniProt
View information about AU1 on UniProt.org
Go To Top Version: 7    Revision Date: 09/20/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • Purified anti-AU1 Epitope Tag

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