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Purified anti-C/EBP β (2 isoforms C/EBP β, LAP) Antibody

Pricing & Availability
Clone
A16 (See other available formats)
Regulatory Status
RUO
Other Names
CCAAT/enhancer-binding protein beta, Alpha-1-acid glycoprotein/enhancer binding protein, Interleukin 6-dependent DNA-binding protein (IL-6DBP), Nuclear factor of interleukin 6 (NF-IL6), Liver-enriched transcriptional activator protein (LAP)
Isotype
Mouse IgG1, κ
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Product Citations
publications
A16_PURE_CslashEBP_Antibody_WB_062618
Total lysates (15 µg protein) from P388D1 (lane 1) and NIH3T3 (lane 2) cells were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with 1 µg/mL Purified anti-C/EBP β antibody (1:500 dilution), clone A16 (upper blot). Proteins were visualized by chemiluminescence detection using a 1:3000 diluted goat anti-mouse-IgG secondary antibody conjugated to HRP for the anti-C/EBP β antibody or 1:5000 diluted Direct-Blot HRP anti-β-Actin antibody, clone 2F1-1 (lower). Lane M: Molecular weight ladder.
  • A16_PURE_CslashEBP_Antibody_WB_062618
    Total lysates (15 µg protein) from P388D1 (lane 1) and NIH3T3 (lane 2) cells were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with 1 µg/mL Purified anti-C/EBP β antibody (1:500 dilution), clone A16 (upper blot). Proteins were visualized by chemiluminescence detection using a 1:3000 diluted goat anti-mouse-IgG secondary antibody conjugated to HRP for the anti-C/EBP β antibody or 1:5000 diluted Direct-Blot HRP anti-β-Actin antibody, clone 2F1-1 (lower). Lane M: Molecular weight ladder.
Cat # Size Price Save
626001 25 µg ¥22,220
626002 100 µg ¥47,960
Description

C/EBPβ (also known as CCAAT/enhancer-binding protein beta, interleukin 6-dependent DNA-binding protein, and nuclear factor of interleukin 6 [NF-IL6]) is a member of the basic leucine zipper C/EBP transcription factor family. Mouse C/EBP has three isoforms that include C/EBPβ-FL, C/EBPβ-LAP, and C/EBPβ-LIP (31kD, 29kD and 16kD, respectively). This protein is localized in the nucleus and functions as a transcription factor in immune, inflammatory, acute-phase, cytokine, collagen type I gene expression. It is upregulated by growth hormones and has been shown to interact with CEBP-α, CEBP-δ, CEBP-γ, CEBP-ε, and CEBP-ζ. The C/EBPβ protein is known to be modified by phosphorylation and ubiquitination. Clone A16 has been shown to be useful for Western blotting to recognize C/EBPβ-FL (31kD) and C/EBPβ-LAP (29kD) isoforms.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant full length mouse C/EBP β protein
Formulation
This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. Final antibody concentration is 0.5 mg/ml.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
Upon receipt, store between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 1.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

A16 monoclonal antibody recognizes C/EBPß-FL (31kD) and C/EBPß-LAP (29kD) isoforms, but not C/EBPß-LIP (16kD).

Application References

(PubMed link indicates BioLegend citation)
  1. Lee YM, et al.1996. Mol. Cell. Biol. 16:4257
  2. Su, et al. 2003. J. Biol. Chem. 278:51150.
Product Citations
  1. Adayev T, et al. 2014. BMC Med Genet. 119:15. PubMed
  2. Brina D, et al. 2015. Nat Commun. 6: 8261. PubMed
RRID
AB_2097741 (BioLegend Cat. No. 626001)
AB_2097741 (BioLegend Cat. No. 626002)

Antigen Details

Structure
C/EBP transcription factor family, basic leucine zipper, dimer. Mouse C/EBP has three isoforms that include C/EBPβ-FL, C/EBPβ-LAP, and C/EBPβ-LIP (31kD, 29kD and 16kD, respectively).
Distribution

Nuclear

Function
Transcription factor in immune, inflammatory, acute-phase, cytokine, collagen type I genes
Interaction
CEBP-α, CEBP-β, CEBP-δ, CEBP-γ, CEBP-ε, CEBP-ζ
Modification
Phosphorylation, Ubiquitination
Biology Area
Cell Biology, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. Chang C, et al. 1990. Mol. Cell Biol. 10:6642.
2. Eaton E, et al. 2001. J. Cell Physiol. 189:91.
3. Piwien-Pilipuk G, et al. 2002. J. Biol. Chem. 277:44557.
4. Hattori T, et al. 2003. Oncogene. 22:1273.

Regulation
Growth hormone
Gene ID
12608 View all products for this Gene ID
UniProt
View information about C/EBP beta on UniProt.org

Related FAQs

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Go To Top Version: 3    Revision Date: 12/17/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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